刺芒柄花素通过FAK/AKT/Bcl-2通路促进外阴鳞癌SW962细胞凋亡  

Formononetin promotes apoptosis in vulvar squamous cell carcinoma SW962 cells through the FAK/AKT/Bcl-2 pathway

作　　者：张珊珊[1] 刘嗣同 石洪爽 关一夫[2] 杨劭劼 武昕[1] ZHANG Shan-shan;LIU Si-tong;SHI Hong-shang;GUAN Yi-fu;YANG Shao-jie;WU Xin(Department of Gynecology,The First Affiliated HospitalofChina Medical University,Shenyang 110001;Department of Biochemistry and Molecular Biology of China Medical University,Shenyang 110022;The Second Affiliated General Surgery Department of Shengjing Hospital of China Medical University,Shenyang 110004,China)

机构地区：[1]中国医科大学附属第一医院妇科,辽宁沈阳110001 [2]中国医科大学生物化学与分子生物学教研室,辽宁沈阳110122 [3]中国医科大学附属盛京医院第二普通外科,辽宁沈阳110004

出　　处：《解剖科学进展》2021年第6期653-656,共4页Progress of Anatomical Sciences

基　　金：国家自然科学基金(30973190)。

摘　　要：目的探讨刺芒柄花素(FMN)对外阴鳞癌SW962细胞凋亡的影响及其相关机制。方法通过CCK8方法和流式细胞仪检测FMN对SW962细胞增殖和凋亡的影响;Westernblot方法检测FMN对细胞凋亡和FAK通路相关蛋白表达的影响;通过EGF激活FAK通路,检测FMN对激活后FAK通路和凋亡相关蛋白表达的影响。结果FMN呈浓度依赖性抑制SW962细胞增殖,促进SW962细胞凋亡;FMN上调Bax、Cleaved-caspase3表达,下调pFAK、p-AKT、Bcl-2表达。EGF处理后p-FAK、p-AKT、Bcl-2表达上调,Bax、Cleaved-caspase3表达下调,FMN可逆转EGF的作用。结论刺芒柄花素通过FAK/AKT/Bcl-2通路促进外阴鳞癌SW962细胞凋亡。Objective To investigate formononetin effect on apoptosis in vulvar squamous cell carcinoma SW962 cells. Methods Formononetin effect on the proliferation and apoptosis of SW962 cells was detected by CCK8 and flow cytometry methods. The expression of apoptosis related proteins and FAK pathway proteins was examed through western-blot method. The same as protein examination after EGF treatment. Results Formononetin inhibited SW962 cells proliferation and promoted apoptosis in a concentration-dependent manner. Formononetin upregulated the expression of Bax and Cleaved-caspase3 proteins and downregulated the expression of p-FAK, p-AKT and Bcl-2 proteins. Bax and Cleavedcaspase3 protein expression was decreased and p-FAK, p-AKT and Bcl-2 protein expression was increased after EGF treatment. Formononetin reversed the effect of EGF. Conclusion Formononetin promotes apoptosis of vulvar squamous cell carcinoma SW962 cells through the FAK/AKT/Bcl-2 pathway.

关 键 词：外阴鳞癌 刺芒柄花素 FAK AKT 细胞凋亡 

分 类 号：R737.35[医药卫生—肿瘤]