METTL3促进HT29和LOVO细胞增殖和迁移的实验研究  

METTL3 promoted proliferation and migration/invasion in HT29 and LOVO cells

作　　者：李达 魏晰麟[2] 石三保 王小磊 张成 LI Da;WEI Xi-lin;SHI San-bao;WANG Xiao-lei;ZHANG Cheng(Department of General Surgery,Northern Theater Command General Hospital,Shenyang 110016;Third Department of General Surgery,Central Hospital Affiliated to Shenyang Medical College,Shenyang 110016,China)

机构地区：[1]北部战区总医院普通外科,辽宁沈阳110016 [2]沈阳医学院附属中心医院普外三科,辽宁沈阳110024

出　　处：《解剖科学进展》2021年第6期701-704,708,共5页Progress of Anatomical Sciences

基　　金：辽宁省自然科学基金面上项目(原优秀人才培育项目20170540951)。

摘　　要：目的研究甲基转移酶样蛋白3(methyltransferase like 3,METTL3)在结直肠癌(colorectal cancer,CRC)组织及细胞系中表达及其对HT29和LOVO细胞增殖和迁移能力的影响。方法应用GEO2R软件分析METTL3在Gene Expression Omnibus (GEO)数据库中结直肠癌相关数据集GSE41258中的表达;免疫组织化学检测METTL3在结肠癌及对应的癌旁组织中的表达;实时荧光定量PCR(RT-qPCR)和蛋白印迹法检测METTL3在细胞学水平的表达;在HT29和LOVO细胞中转染特异性METTL3小干扰RNA(siMETTL3)及相应对照(siNC),克隆形成实验及Transwell实验检测HT29和LOVO细胞增殖及迁移侵袭能力的变化。结果在GSE41258中,METTL3在结肠癌组织中的表达明显高于其在正常结直肠组织中的表达;METTL3在收集的结直肠癌组织中的表达高于相应的癌旁组织;相比于正常肠上皮细胞系NCM460,METTL3 mRNA在结直肠癌细胞系中的表达升高;在HT29和LOVO细胞中转染siMETTL3后,METTL3的蛋白水平明显下调;下调METTL3的表达可明显抑制HT29和LOVO细胞的增殖、迁移和侵袭能力。结论 METTL3在结直肠癌组织及细胞系中表达增高,在HT29和LOVO细胞中下调METTL3的表达可明显抑制细胞的增殖、迁移及侵袭能力。Objective The current research aims to identify the expression of methyltransferase like 3(METTL3). Also, the current study aims to illustrate the effect of METTL3 on proliferation and migration in HT29 and LOVO cells. Methods An online software GEO2 R was used to analyze the expression of METTL3 in GSE41258. Whilst, the expression of METTL3 in CRC and paired paratumor tissue was determined by immunohistochemistry(IHC). Reverse transcription-quantitative polymerase chain reaction(RT-qPCR) and western blot was applied to detect the expression of METTL3 in cellular level. METTL3 small interfering RNA(siMETTL3) and corresponding negative control small interfering RNA(siNC) were transfected into HT29 and LOVO cells. The proliferation and migration ability changes of HT29 and LOVO cells were verified by colony formation assay and transwell assay. Results In GSE41258, METTL3 was highly expressed in CRC tissues as compared with that in normal colorectal tissues. METTL3 was upregulated in our collected CRC tissues as compared with that in paratumor tissues. METTL3 mRNA was upregulated in CRC cell lines as comparing with that in a normal colon epithelial cell line NCM460. Transfection of siMETTL3 suppressed METTL3 protein expression in HT29 and LOVO cells. Downregulation of METTL3 significantly inhibited proliferation and migration abilities of HT29 and LOVO cells. Conclusion METTL3 was upregulated in CRC, and downregulation of METTL3 inhibited migration and proliferation of HT29 and LOVO cells.

关 键 词：甲基转移酶样蛋白3 结直肠癌 增殖 迁移 侵袭 

分 类 号：R735.3[医药卫生—肿瘤]