丹酚酸B对肝癌荷瘤小鼠抑瘤作用及对肝纤维化的影响  

Salvianolic Acid B on Tumor-inhibiting Effect of Hepatocarcinoma-bearing Mice and Its Effect on Liver Fibrosis Expression

作　　者：肖珊珊[1] 张卓尔 杨巧红[3] 李建婷[3] 季湍云 XIAO Shan-shan;ZHANG Zhuo-er;YANG Qiao-hong;LI Jian-ting;JI Tuan-yun(I Department of Pathology,First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine,Guangzhou,Guangdong,510405,China;Department of Clinical Medicine,Southern Medical University,Guangzhou,Guangdong,518056,China;Department of Pathology,School of Basic Medicine,Guangzhou University of Chinese Medicine,Guangzhou,Guangdong,510006,China)

机构地区：[1]广州中医药大学第一附属医院病理科,广东广州510405 [2]南方医科大学临床医学系,广东广州518056 [3]广州中医药大学基础医学院病理教研室,广东广州510006

出　　处：《现代生物医学进展》2021年第22期4207-4210,4248,共5页Progress in Modern Biomedicine

基　　金：广东省中医药局科研项目(20211122)。

摘　　要：目的:探讨丹酚酸B(salvianolic acid B,Sal B)对肝癌荷瘤小鼠抑瘤作用及对肝纤维化的影响。方法:将HepG2肝癌细胞株皮下注射于裸鼠左腋下成瘤,将荷瘤小鼠(n=42)随机平分为三组:模型组、Sal B 1组与Sal B 2组。Sal B 1组与Sal B 2组于造模成功当天开始分别给予10 m L/kg/d和15 m L/kg/d丹酚酸B进行灌胃,模型组给予等量生理盐水灌胃,每周2次,连续4周。结果:治疗第2周与第4周后,Sal B 1组、Sal B 2组的移植瘤重量均低于模型组(P<0.05),抑瘤率高于模型组(P<0.05),Sal B 1组与Sal B 2组对比差异有统计学意义(P<0.05)。Sal B 1组、Sal B 2组肝脏系数与肝脏表面癌结节数目均低于模型组(P<0.05),Sal B 2组低于Sal B 1组(P<0.05)。Sal B 1组、Sal B 2组血清天门冬氨酸氨基转移酶(Aspartate transaminase,AST)、丙氨酸氨基转移酶(Alanine transaminase,ALT)含量均低于模型组(P<0.05),Sal B 2组低于Sal B 1组(P<0.05)。Sal B 1组、Sal B 2组肝组织转化生长因子-β1(Transforming growth factor-beta 1,TGF-β1)、Smad3蛋白相对表达水平均低于模型组(P<0.05),Sal B 2组低于Sal B 1组(P<0.05)。结论:Sal B在肝癌荷瘤小鼠中能发挥抑瘤作用,可抑制血清ALT与AST的释放,其可能是通过TGF-β1/Smad3信号发挥抗肝纤维化-肝癌作用。Objective:To investigate the tumor-inhibiting effect of salvianolic acid B(Sal B)on liver cancer tumor-bearing mice and its influence on the expression of liver fibrosis.Methods:Mice bearing liver cancer(n=42)were randomly divided into three groups-model group,Sal B 1 group and Sal B 2 group.Sal B 1 group and Sal B 2 group were separately given 10 m L/kg/d and 15 m L/kg/d salvianolic acid B by gavage on the day of modeling,and the model group were given the same amount of normal saline by gavage twice a week for 4 consecutive weeks.Results:The weight of the transplanted tumors in the Sal B 1 group and Sal B 2 group were lower than that of the model group(P<0.05)in the 2 nd and 4 th weeks of treatment,and the tumor inhibition rate were higher than that of the model group(P<0.05),and compared the difference between the Sal B 1 group and the Sal B 2 group were also statistically significant(P<0.05).The liver coefficient and the number of liver surface cancer nodules in the Sal B 1 group and Sal B 2 group were lower than the model group(P<0.05)in the second and fourth weeks of treatment,and the Sal B 2 group were also lower than the Sal B 1 group(P<0.05).The serum aspartate transaminase(AST)and alanine transaminase(ALT)levels of the Sal B 1 group and Sal B 2 group were lower than the model in the 2 nd and 4 th weeks of treatment Group(P<0.05),Sal B 2 group were also lower than Sal B 1 group(P<0.05).The relative expression levels of transforming growth factor-β1(TGF-β1)and Smad3 protein in liver tissues in the 2 nd and 4 th weeks of treatment in the Sal B 1 group and Sal B 2 group were lower than those in the model group(P<0.05),the Sal B 2 group were also lower than the Sal B1 group(P<0.05).Conclusion:The application of salvianolic acid B in hepatocarcinoma-bearing mice can inhibit tumors and inhibit the release of serum ALT and AST,which may be through the TGF-β1/Smad3 signal to play anti-liver fibrosis-liver cancer effect.

关 键 词：丹酚酸B 肝癌 肝纤维化 天门冬氨酸氨基转移酶 转化生长因子-Β1 肝脏系数 

分 类 号：R-33[医药卫生] R735.7