环状RNA circ0004771对肺腺癌细胞A549的增殖、迁移与侵袭的影响  

Effects of circRNA circ0004771 on the proliferation,migration,and invasion of lung adenocarcinoma cell line A549

作　　者：杨露 丁剑桥 陈金鑫 张叶 张亮 项博文 秦宝丽 Yang Lu;Ding Jianqiao;Chen Jinxin;Zhang Ye;Zhang Liang;Xiang Bowen;Qin Baoli(Department of Internal Medicine,Cancer Hospital of China Medical University,Liaoning Cancer Hospital&Institute,Shenyang 110042,China;Department of Thoracic Surgery,Cancer Hospital of China Medical University,Liaoning Cancer Hospital&Institute,Shenyang 110042,China;Department of Gynecology,Cancer Hospital of China Medical University,Liaoning Cancer Hospital&Institute,Shenyang 110042,China;Department of Radiation Oncology,Cancer Hospital of China Medical University,Liaoning Cancer Hospital&Institute,Shenyang 110042,China)

机构地区：[1]中国医科大学肿瘤医院辽宁省肿瘤医院肿瘤内科,沈阳110042 [2]中国医科大学肿瘤医院辽宁省肿瘤医院胸外科,沈阳110042 [3]中国医科大学肿瘤医院辽宁省肿瘤医院妇科,沈阳110042 [4]中国医科大学肿瘤医院辽宁省肿瘤医院肿瘤放疗科,沈阳110042

出　　处：《中华解剖与临床杂志》2022年第1期42-47,共6页Chinese Journal of Anatomy and Clinics

摘　　要：目的探讨环状RNA circ0004771对肺腺癌细胞增殖、迁移与侵袭的影响及其机制。方法培养肺腺癌A549细胞,分为阴性对照(si-NC)组及circ0004771小干扰RNA(siRNA)组(si-circ0004771组),将si-NC和circ0004771 siRNA分别转染入相应组A549细胞中。si-NC组和si-circ0004771组细胞转染后,通过实时荧光定量聚合酶链反应检测2组细胞circ0004771、miR-339-5p的表达水平,通过细胞计数试剂盒法检测细胞活力,通过EdU实验检测2组细胞增殖能力,通过Transwell小室实验检测2组细胞迁移和侵袭能力。结果si-NC组和si-circ0004771组A549细胞circ0004771基因的相对表达水平分别为3.07±0.07和0.68±0.04,miR-339-5p相对表达水平分别为1.14±0.13和2.33±0.07,差异均有统计学意义(t=51.34、13.96,P值均<0.001)。si-NC组和si-circ0004771组A549细胞在培养前(0 h)和培养后24、48 h的吸光度值分别为0.21±0.02、0.35±0.05、0.65±0.04和0.21±0.01、0.33±0.04、0.59±0.05,差异均无统计学意义(P值均>0.05);而在培养后72 h si-circ0004771组吸光度为0.92±0.15,小于si-NC组的1.32±0.04,差异有统计学意义(t=4.46,P=0.011)。EdU实验中,si-circ0004771组细胞阳性率为47.97%±4.68%,低于si-NC组的58.61%±2.15%,差异有统计学意义(t=3.58,P=0.023)。转染后si-circ0004771组的细胞迁移率、侵袭率分别为52.27%±2.92%、55.33%±6.29%,均低于si-NC组的99.79%±4.23%、98.67%±5.72%,差异均有统计学意义(t=16.26、8.83,P值均<0.001)。结论下调肺腺癌A549细胞circ0004771的表达水平,可以降低A549细胞的活力和增殖能力,可抑制A549细胞的迁移、侵袭能力,其作用机制可能与上调miR-339-5p的表达有关。Objective To investigate the biological role of circ0004771 in lung adenocarcinoma.Methods Lung adenocarcinoma A549 cells were cultured and divided into the negative control(si-NC)and circ0004771 siRNA(si-circ0004771)groups.Si-NC and circ0004771 siRNA were transfected into A549 cells in the corresponding groups.After the transfection of the cells in the si-NC and si-circ0004771 groups,the expression levels of circ0004771 and miR-339-5p were detected via real-time fluorescent quantitative polymerase chain reaction;cell viability was determined through cell counting kit-8;cell proliferation was detected by using the EdU assay;and cell migration and invasion were detected through the Transwell chamber assay.Results In the A549 cells in the si-NC and si-circ0004771 groups,the relative expression levels of the circ0004771 gene were 3.07±0.07 and 0.68±0.04,respectively,and the relative expression levels of miR-339-5p were 1.14±0.13 and 2.33±0.07,respectively;these differences were statistically significant(t=51.34,13.96,all P values<0.001).The absorbance values of the A549 cells in the si-NC and si-circ0004771 groups at 0,24,and 48 h were 0.21±0.02,0.35±0.05,and 0.65±0.04,respectively,and 0.21±0.01,0.33±0.04,and 0.59±0.05,respectively;no significant difference was observed(all P values>0.05).However,at 72 h,the absorbance of the si-circ0004771 group was 0.92±0.15,which was less than that of the si-NC group(1.32±0.04);this difference was statistically significant(t=4.46,P=0.011).In the EdU experiment,the positive rate of cells in the si-circ0004771 group was 47.97%±4.68%,which was lower than the positive rate of 58.61%±2.15% shown by the si-NC group;this difference was statistically significant(t=3.58,P=0.023).After transfection,the cell migration and invasion rates in the si-circ0004771 group were 52.27%±2.92% and 55.33%±6.29%,respectively,and were all lower than the cell migration and invasion rates of 99.79%±4.23% and 98.67%±5.72% in the si-NC group;this difference was statistically significant(t=16

关 键 词：肺肿瘤 肺腺癌 A549细胞株 细胞增殖 细胞迁移 细胞侵袭 

分 类 号：R734.2[医药卫生—肿瘤]