两个产地洋葱的UHPLC图谱分析及前列腺素A1含量测定  

UHPLC profiling of onions from two origins and the determination of prostaglandin A1 content

作　　者：王晓婷 谭超[1] 王燕华 秦太峰 张乃明 李芳艳[3] WANG Xiao-ting;TAN Chao;WANG Yan-hua;QIN Tai-feng;ZHANG Nai-ming;LI Fang-yan(College of Food Science and Technology,Yunnan Agricultural University,Kunming,Yunnan 650201,China;Yunnan Soil Fertilizer and Pollution Remediation Engineering Laboratory,Kunming,Yunnan 650201,China;College of Resource and Environment,Yunnan Agricultural University,Kunming,Yunnan 650201,China)

机构地区：[1]云南农业大学食品科学技术学院,云南昆明650201 [2]云南省土壤培肥与污染修复工程实验室,云南昆明650201 [3]云南农业大学资源与环境学院,云南昆明650201

出　　处：《食品与机械》2022年第1期44-51,共8页Food and Machinery

基　　金：黑龙江齐齐哈尔地方政府合作项目(编号:KX13190250001)。

摘　　要：目的:对不同产地洋葱化学成分含量的差异进行分析评价。方法:以中国南北(云南和黑龙江)两个主产地的洋葱为原料,采用超高效液相色谱(UHPLC),结合多元统计学分析两个产地洋葱化学成分含量差异及特征化学成分前列腺素A1(PGA1)。结果:洋葱UHPLC化学成分含量图谱共标定73个共有色谱峰,通过正交偏最小二乘法,能区别出两个产地洋葱化学成分含量,结合VIP值>1、P<0.05和S-plot分析共筛选出11种主要差异标志物。云南洋葱PGA1含量[(197.21±63.22)mg/kg]显著高于黑龙江洋葱[(121.37±43.12)mg/kg]。结论:云南和黑龙江两个产地洋葱化学成分含量及PGA1含量存在显著差异。Objective:This study focused on the analysis and evaluation of the differences in the chemical composition of onions from different origins.Methods:Onions from two main production areas in North and South China(Yunnan and Heilongjiang)were used as raw materials,and the differences in chemical composition content and characteristic chemical composition prostaglandin A1 between the two production areas were analyzed by ultra-high performance liquid chromatography(UHPLC)combined with multivariate statistics.Results:A total of 73 peaks were identified by UHPLC,and the chemical contents of onions from two origins could be distinguished by orthogonal partial least square discriminant analysis(OPLS-DA),combined with VIP values>1,P<0.05 and S-plot.0.05 and S-plot analysis,and a total of 11 major markers of variation were screened.The PGA1 content of Yunnan onion[(197.21±63.22)mg/kg]was significantly higher than that of Heilongjiang onion[(121.37±43.12)mg/kg].Conclusion:There were significant differences in the chemical composition content and PGA1 content of onion between the two origins in Yunnan and Heilongjiang.

关 键 词：洋葱 前列腺素 超高效液相色谱 

分 类 号：O657.72[理学—分析化学] TS207.3[理学—化学]