不同碳氮源对花脸香蘑胞外酶活性的影响  

Effects of different carbon and nitrogen sources on extracellular enzyme activities of Lepista sordida

作　　者：陆欢[1,2] 王小艳 尚晓冬 王瑞娟[1] 徐宁[2] LU Huan;WANG Xiao-Yan;SHANG Xiao-Dong;WANG Rui-Juan;XU Ning(Key Laboratory of Applied Fungal Resources and Utilization(South),Ministry of Agriculture of China,National Engineering Research Center of Edible Fungi,Shanghai Key Laboratory of Agricultural Genetics and Breeding,Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Shanghai 201403,China;Institute of Hunan Edible Fungi,Changsha,Hunan 410013,China)

机构地区：[1]上海市农业科学院食用菌研究所,农业农村部应用真菌资源与利用重点开放实验室,囯家食用菌工程技术研究中心,上海市农业遗传育种重点实验室,上海201403 [2]湖南省食用菌研究所,湖南长沙410013

出　　处：《菌物学报》2021年第12期3286-3303,共18页Mycosystema

基　　金：湖南省自然科学基金(2019JJ50330);上海市农业科学院卓越团队建设计划[2017(A-02)]。

摘　　要：以花脸香蘑Lepista sordida为材料,研究其分别在9种碳源和11种氮源液体培养条件下不同阶段pH值和葡萄糖浓度的变化,以及不同碳氮源对其所分泌的木质素过氧化物酶、羧甲基纤维素酶、锰过氧化物酶和漆酶活性的影响。结果表明,pH值在不同碳源培养后期变化显著(P<0.05),而在不同氮源培养阶段无明显变化(P>0.05),葡萄糖浓度和菌丝量在不同碳氮源中也无显著差异(P<0.05)。不同碳源和氮源培养基对花脸香蘑木质素过氧化物酶、羧甲基纤维素酶、锰过氧化物酶和漆酶活性均具有影响(P<0.05)。木糖和米糠有利于花脸香蘑分泌羧甲基纤维素酶(P<0.05),红糖和牛肉浸膏有利于其分泌漆酶(P<0.05),白砂糖和豆粉有利于其分泌锰过氧化物酶(P<0.05),木糖和尿素有利于其分泌木质素过氧化物酶(P<0.05)。本研究为选择合适培养基以提高花脸香蘑生物转化效率提供了理论基础。Lepista sordida was used as experimental material to study the variation of pH-value and glucose concentration and lignin peroxidase,carboxymethyl cellulase,manganese peroxidase,and laccase activities in liquid cultures containing different carbon and nitrogen sources(9 carbon and 11 nitrogen sources).The results showed that the pH-value changed significantly in the later period of culture using different carbon sources(P<0.05),but there was no significant change in the cultures with different nitrogen sources(P>0.05).The glucose concentration and mycelial biomass were not significantly different in the cultures with different carbon and nitrogen sources(P<0.05).The activities of lignin peroxidase,carboxymethyl cellulase,manganese peroxidase and laccase varied with different carbon source and nitrogen sources(P<0.05).Xylose and rice bran were conducive to the secretion of carboxymethyl cellulase(P<0.05),and brown sugar and beef extract were conducive to the secretion of laccase(P<0.05);white granulated sugar and bean powder were conducive to the secretion of manganese peroxidase(P<0.05),and xylose and urea were conducive to the secretion of lignin peroxidase(P<0.05).This study provided a reference for the selection of appropriate medium to improve the efficiency of biotransformation of L.sordida.

关 键 词：花脸香蘑 碳氮源 胞外酶 pH 值 菌丝生物量 

分 类 号：S646[农业科学—蔬菜学]