菜薹和芥蓝种间杂交胚挽救技术研究  

Embryo Rescue of Interspecific Brassica campestris x B.albograbra Hybrids

作　　者：吕凤仙 李崇娟 杨鼎 胡靖锋[2,3] 杨红丽 兰梅[2,3] 徐学忠 张丽琴[2,3] 和江明 董相书[1] LYU Fengxian;LI Chongjuan;YANG Ding;HU Jingfeng;YANG Hongli;LAN Mei;XU Xuezhong;ZHANG Liqin;HE Jiangming;DONG Xiangshu(Yunnan University,Kunming,Yunnan 650504,China;Horticultural Crops Research Institute,Yunnan Academy Agricultural Science,Kunming,Yunnan 650205,China;Yunnan Branch of National Vegetable Improvement Center,Kunming,Yunnan 650205,China)

机构地区：[1]云南大学,云南昆明650504 [2]云南省农业科学院园艺作物研究所,云南昆明650205 [3]国家蔬菜改良分中心云南分中心,云南昆明650205

出　　处：《福建农业学报》2021年第11期1288-1294,共7页Fujian Journal of Agricultural Sciences

基　　金：云南省“万人计划”产业领军人才项目(YNWR-CYJS-2018-040);国家大宗蔬菜产业技术体系昆明综合试验站项目(CARS-23-G37);云南省科技计划重大科技专项(2019ZG001)。

摘　　要：【目的】获得适合菜薹和芥蓝种间杂种的胚挽救实验方案。【方法】以菜薹(菜心)作为母本,芥蓝作为父本,进行种间杂交,对种间杂种进行人工胚挽救培养,设置8种不同成分的胚挽救培养基,6个不同取材时间,4种增殖培养基,9种不同成分生根培养基。【结果】ERM4(MS+6-BA 0.5 mg·L^(−1)+NAA 0.5 mg·L^(−1)+KT 1.0 mg·L^(−1)+AC 1.0 g·L^(−1))出胚率达26.32%,为最佳胚挽救培养基;授粉后第12天取材,出胚率达26.32%,为最适宜取材时间;DM1(MS+6-BA 0.9 mg·L^(−1)+NAA 0.1 mg·L^(−1))培养基增殖系数最大,为5.37,为最佳增殖培养基;RM4-2(1/2MS+IBA0.5 mg·L^(−1))培养基生根率为37.21%,平均生根时间为8.33 d,生根最快,为最佳生根培养基。【结论】菜薹与芥蓝种间杂交,于授粉后12 d取材,胚珠接种在ERM4培养基上,出胚后接种在DM1培养基上增殖,在RM4-2培养基上生根,能够显著提高种间杂种的获得率。【Objective】Methods suitable for embryo rescue in the interspecific hybridization between Brassica campestris and B.albograbra were investigated.【Method】The interspecific crossing was carried out using B.campestris L.as female parent and B.albograbra L.H.Bailey as male parent.An embryo rescue culture for the hybridization was conducted with 8 different media at 6 sampling times followed subsequently by 4 proliferation media and 9 rooting media.【Result】The embryo genesis rate at 26.32%of ERM4 medium consisting of MS,0.5mg 6-BA·L^(−1),0.5mg NAA·L^(−1),1.0mg KT·L^(−1),and 1.0g AC·L^(−1) was the highest among all.The samples collected 12d after pollination had the best rate of 26.32%,DM1(i.e.,MS+0.9mg 6-BA·L^(−1)+0.1mg NAA·L^(−1))the greatest proliferation coefficient of 5.37,and RM4-2(i.e.,1/2MS+0.5mg IBA·L^(−1))the top rate of 37.21%and average 8.33d on rooting.【Conclusion】The optimum conditions for the embryo rescue in the interspecific hybridization between B.campestris L.and B.albograbra L.H.Bailey included sampling at 12d after pollination and following by culture on ERM4 medium for embryo induction,on DM1 for ovule proliferation,and on RM4-2 for seedling rooting.

关 键 词：菜薹 芥蓝 种间杂交 胚挽救 增殖 不定根诱导 

分 类 号：S634[农业科学—蔬菜学]