铜离子对滇水金凤花色变化的生理生化研究  

Physiological and Biochemical Correlations between Color of Impatiens uliginosa Flower and Nutrient Supply on Copper

作　　者：李芹梅 李文祥 曹孟会 刘松 张天谣 汪琼[1] 黄美娟[1] 黄海泉[1] LI Qinmei;LI Wenxiang;CAO Menghui;LIU Song;ZHANG Tianyao;WANG Qiong;HUANG Meijuan;HUANG Haiquan(College of Landscape Architecture and Horticulture Sciences/Southwest Research Center for Engineering Technology of Landscape Architecture(State Forestry and Grassland Administration)/Yunnan Engineering Research Center for Functional Flower Resources and Industrialization/Research and Development Center of Landscape Plants and Horticulture Flowers,Southwest Forestry University,Kunming Yunnan 650224,China)

机构地区：[1]西南林业大学园林园艺学院/国家林业和草原局西南风景园林工程技术研究中心/云南省功能性花卉资源及产业化技术工程研究中心/西南林业大学园林园艺花卉研发中心,云南昆明650224

出　　处：《福建农业学报》2021年第11期1323-1329,共7页Fujian Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(32060364、32060366、31860230);云南省重点研发计划项目(2018BB013);云南省教育厅科学研究基金项目(2021Y262);云南省中青年学术和技术带头人培养项目(2015HB046、2018HB024);云南省高校园林植物与观赏园艺科技创新团队项目(51700204)。

摘　　要：【目的】探讨滇水金凤花色对重金属铜离子的响应。【方法】以滇水金凤为试验材料,研究其在不同含量Cu^(2+)(0、5、10、15、20 mg·L^(−1))处理下盛花期花瓣的色度值、细胞液pH值、色素和基础代谢物含量的变化,并分析Cu^(2+)、各生理生化指标与花色之间的关系。【结果】(1)花色苷、总黄酮和可溶性糖含量随Cu^(2+)含量增加而下降,与对照比均达显著差异性(P<0.05);(2)随Cu^(2+)含量增加,可溶性蛋白含量不断增加,与对照比均达显著差异性(P<0.05);(3)Cu^(2+)含量在0~15 mg·L^(−1)时,类胡萝卜素和脯氨酸含量无明显变化;(4)相关性分析表明,不同含量Cu^(2+)处理下花瓣色度值、花色苷、总黄酮、可溶性糖、脯氨酸、可溶性蛋白等几个指标间均存在极显著相关性;(5)运用逐步回归分析发现,色相a*值、b*值主要受花色苷、可溶性糖和脯氨酸等因子影响。【结论】花色苷含量是影响滇水金凤花色变化的直接因素;而Cu^(2+)是通过调控滇水金凤花色苷的生物合成来影响花瓣呈色。【Objective】Response of Impatiens uliginosa to copper ions on the floral color with respect of the physiological and biochemical properties of the petals was investigated.【Method】Under treatments of varied Cu^(2+)concentrations(i.e.,0,5,10,15,and 20 mg·L^(−1))in culture medium,the chromaticity,pH of cell fluid,pigments,and basal metabolites of flower petals of the I.uliginosa at full blooming stage were determined.The correlations among Cu^(2+),physiological and biochemical indices,and flower color were analyzed.【Result】(1)The contents of anthocyanins,flavonoids,and soluble sugars in petals decreased significantly in comparison to control as the Cu^(2+)concentration increased(P<0.05).(2)Soluble protein increased significantly over control with increasing Cu^(2+)at P<0.05.(3)In the range of 0-15 mg·L^(−1),Cu^(2+)exerted no significant effect in the contents of carotenoids and proline.(4)There were extremely significant correlations among the color values,anthocyanins,flavonoids,soluble sugar,proline,and soluble protein of petals on the plants treated by Cu^(2+)at different concentrations.(5)The stepwise regression analysis showed that the flower chromaticity a*and b*were mainly affected by anthocyanins,soluble sugar and proline.【Conclusion】Anthocyanin appeared to directly govern the color of I.uliginosa flowers.Cu^(2+)seemed to affect the petal color through regulating anthocyanin biosynthesis in I.uliginosa.

关 键 词：滇水金凤 Cu^(2+) 花色 色素 基础代谢物 

分 类 号：S681.1[农业科学—观赏园艺]