茶树Gro/Tup1基因家族鉴定及外源激素和非生物胁迫下表达分析  

Genome-wide Identification of Gro/Tup1 Gene Family in Camellia sinensis and Their Expression Analysis Under Exogenous Phytohormones and Abiotic Stress Treatments

作　　者：詹冬梅 朱晨[1,2] 周承哲 黄雪婷 赖钟雄 郭玉琼[1,2] ZHAN Dong-mei;ZHU Chen;ZHOU Cheng-zhe;HUANG Xue-ting;LAI Zhong-xiong;GUO Yu-qiong(College of Horticulture/Key Laboratory of Tea Science of Fujian Province/Tea Industry Research Institute,Fujian Agriculture and Forestry University,Fuzhou 350002;Institute of Horticultural Biotechnology,Fujian Agriculture and Forestry University,Fuzhou 350002)

机构地区：[1]福建农林大学园艺学院/茶学福建省高校重点实验室/茶产业研究院,福州350002 [2]福建农林大学园艺植物生物工程研究所,福州350002

出　　处：《生物技术通报》2021年第12期1-12,共12页Biotechnology Bulletin

基　　金：福建农林大学“双一流”建设科技创新能力提升培育计划(KSYLP004);6.18协同创新院茶产业技术分院专项(K1520001A);福建农林大学茶产业链科技创新与服务体系建设项目(K1520005A01);福建省高原学科建设经费(102/71201801101);福建农林大学科技创新专项基金项目(CXZX2017537)。

摘　　要：明确Groucho/Thymidine uptake 1(Gro/Tup1)辅转录调控因子在茶树基因组中的数量、结构及其在非生物胁迫和外源激素处理下表达差异,为揭示Gro/Tup1在茶树激素信号转导途径及其在茶树逆境胁迫中的作用奠定理论基础。对茶树Gro/Tup1家族进行鉴定、分析,同时对其编码蛋白理化性质、进化关系、顺式作用元件及其在干旱、低温(4℃)、赤霉素(GA_(3))、脱落酸(ABA)和茉莉酸甲酯(MeJA)处理下表达模式进行分析。结果显示:茶树Gro/Tup1家族具有13个成员,分为TOPLESS/TOPLESS-related(TPL/TPR)和LEUNIG/LEUNIG HOMOLOG(LUG/LUH)两个亚家族。顺式作用元件预测结果表明,茶树Gro/Tup1家族成员启动子区域有大量与GA、ABA、MeJA响应以及干旱和低温应答的顺式作用元件;RT-qPCR分析结果表明,不同处理下,茶树Gro/Tup1家族成员呈现不同表达模式。重度干旱时,CsLUG3和CsLUG4基因的表达量显著上调,CsTPL2表达水平在轻度干旱时就开始受到抑制,CsTPL8和CsTPL9不受干旱调控;低温处理下CsTPL2和CsTPL3表达量显著上调;GA_(3)处理下,多数基因表达量上调;ABA处理下多数基因表达量均呈现先显著增加后显著下降趋势;MeJA处理下,大部分基因表达水平都受到显著抑制。茶树含有13个Gro/Tup1家族成员,其结构进化高度保守,能够响应非生物胁迫及外源激素,其基因应答呈现不同表达谱。The objective of this work is to clarify the number and structure of the co-transcription regulatory factor Groucho/Thymidine uptake 1(CsGro/Tup1)in Camellia sinensis genome,and its expression differences under abiotic stresses and exogenous phytohormones treatments,which will lay a theoretical foundation for revealing the role of Gro/Tup1 in hormone signal transduction pathway and its role in C.sinensis tree stress.The CsGro/Tup1 family members were identified and analyzed.Meanwhile,the physicochemical properties of its encoded proteins,evolutionary relationships,and cis-acting elements of CsGro/Tup1s were investigated,and the relative expression levels of CsGro/Tup1 members were measured under drought,low temperature,GA_(3),ABA and MeJA treatments.The results showed that the CsGro/Tup1 family had 13 members,which were divided into two subfamilies:TOPLESS/TOPLESS-related(TPL/TPR)and LEUNIG/LEUNIG HOMOLOG(LUG/LUH).The prediction results of cis-acting elements showed that Gro/Tupls’promoter region had a large number of cisacting elements in response to GA,ABA,MeJA,drought and low temperature.RT-qPCR results demonstrated that Gro/Tup1s presented different expression patterns under different treatments.In severe drought,the expressions of CsLUG3 and CsLUG4 genes were significantly up-regulated,the expressions of CsTPL2 were inhibited at light drought,CsTPL8 and CsTPL9 were not regulated by drought.The expressions of CsTPL2 and CsTPL3 were significantly up-regulated under low temperature treatment.Under GA_(3) treatment,most gene expressions were upregulated.Under ABA treatment,the expressions of most genes significantly increased in the beginning and significantly decreased later.Most of the gene expressions were significantly inhibited under MeJA treatment.In conclusion,C.sinensis has 13 CsGro/Tup1 family members,and their structures are evolutionarily conservative,and CsGro/Tup1s are able to respond to different abiotic stresses and exogenous phytohormone,and their responses present various profiles.

关 键 词：茶树 Groucho/Thymidine uptake 1(Gro/Tup1)家族 植物激素 非生物胁迫 

分 类 号：S571.1[农业科学—茶叶生产加工]