深海出芽短梗霉(Aureobasidium sp.3A00493)菌株特征与胞外多糖特性分析  

Characteristics of Aureobasidium sp.3A00493 from Deep Sea Sediment and Characteristic Analysis of Its Extracellular Polysaccharide

作　　者：潘镜宇 陈佳乐 钱玙呈 刘鑫[1] 杨昊宁 刘立[1] 魏步云 赵洪新 PAN Jing-yu;CHEN Jia-le;QIAN Yu-cheng;LIU Xin;YANG Hao-ning;LIU Li;WEI Bu-yun;ZHAO Hong-xin(Zhejiang Province Key Laboratory of Plant Secondary Metabolism and Regulation,College of Life Sciences and Medicine,Zhejiang SciTech University,Hangzhou 310018;Department of Bioengineering,Liaoning Technical University,Fuxin 123000)

机构地区：[1]浙江理工大学生命科学与医药学院浙江省植物与次生代谢重点实验室,杭州310018 [2]辽宁工程技术大学生物工程系,阜新123000

出　　处：《生物技术通报》2021年第12期71-81,共11页Biotechnology Bulletin

摘　　要：普鲁兰糖(pullulan)是短梗霉属(Aureobasidium spp.)的一些菌产生的极具高附加值、开发价值的天然微生物多糖。然而不同来源的菌株在产糖量、抑菌活性、副产物含量等方面有很大的差异,因此,寻找产糖量高、抑菌活性强、副产物少的优良菌株一直是研究人员关注的焦点。本研究的出发菌株3A00493是一株从太平洋深海沉积物中分离的产胞外多糖海洋微生物。通过形态学和显微镜观察、分子鉴定确定其分类归属;改变培养条件确定了其生长特性、最佳产糖条件;选择5种常见微生物作为指示菌,滤纸片法测定其抑菌活性;薄层层析和红外光谱分析了多糖结构。出发菌株3A00493是一株菌落乳白,细胞和菌丝体可转换生长,ITS和18S rDNA部分序列与报道短梗霉属(Aureobasidium sp.)同源性≥98%的海洋源出芽短梗霉菌;其最适生长温度为26℃,最适生长pH值为4,最适条件下产糖量达24.58 g/L;3A00493的代谢产物对4种指示菌具有较强抑菌活性;薄层层析和红外光谱测定结果表明3A00493产生的胞外多糖与普鲁兰糖标准品一致,确定为普鲁兰糖。出发菌株3A00493为一株产普鲁兰糖、代谢物抑菌活性较强、色素副产物少的海洋源出芽短梗霉新菌株。本研究为进一步研究海洋源产普鲁兰糖新菌3A00493奠定了理论基础,为寻找海洋源微生物多糖提供了新菌株,也为筛选和开发海洋资源微生物提供研究借鉴。Pullulan is a natural microbial polysaccharide with high developed value produced by fungus Aureobasidium sp.But different strains present great differences in polysaccharide yield,biological activities and by-products.Therefore,it has always been the focus of researchers to find the excellent strains with high sugar production,strong bacteriostatic activity and less by-products.The original strain is an extracellular polysaccharide-producing microorganism isolated from deep seabed sediments in the Pacific Ocean.On the basis of its morphological and microscopic observation as well as molecular identification,the strain was identified as its genus.The growth characteristics and optimal polysaccharide production conditions were determined via changing the culturing conditions.Five common microorganisms were selected as indicator bacteria,and the antibacterial activity was determined by filter paper method,and the structure of polysaccharides was analyzed by thin layer chromatography and infrared spectroscopy.The original strain 3A00493 was marine-sourced Aureobasidium pullulans,its colony was milky white,its cell and mycelium grew in converted way,and its ITS and 18S rDNA partial sequences shared homology of≥98%with reported Aureobasidium sp.Its optimal growth temperature was 26℃and optimal growth pH was 4,under which the polysaccharide yield reached 24.58 g/L.The metabolites of the strain 3A00493 had well antibacterial activity against 4 pathogenic indicator microorganisms.TLC and IR spectra showed that the extracellular polysaccharide produced by the strain 3A00493 was consistent with the pullulan standard sample,thus it was determined as pullulan.In conclusion,original strain 3A00493 is a new marine-sourced A.pullulans producing pullulan,being strong antibacterial activity of its metabolite,and having little pigment byproduct.This work lays a theoretical foundation for further studying marinesourced pullulan-producing 3A00493,providing a new strain in seeking marine-sources microbial polysaccharide,also prov

关 键 词：出芽短梗霉 普鲁兰糖 结构鉴定 抑菌活性 

分 类 号：Q939.9[生物学—微生物学]