人乳寡糖的高分辨质谱结构分析及液相色谱条件的比较  

Structural Analysis of Human Milk Oligosaccharides via High Resolution Mass Spectrometry and Optimization of Chromatographic Separation Conditions

作　　者：王海燕 张曦美 崔秀秀 康鹏 张艳 张佳莹 葛武鹏[1] WANG Hai-Yan;ZHANG Xi-Mei;CUI Xiu-Xiu;KANG Peng;ZHANG Yan;ZHANG Jia-Ying;GE Wu-Peng(College of Food Science and Engineering,Northwest A&F University,Yangling 712100,China;Xi′an Baiyue Goat Group Co.Ltd.,Xi′an 710000,China;Shaanxi Goat Milk Product Quality Supervision and Inspection Center,Fuping 711700,China)

机构地区：[1]西北农林科技大学食品科学与工程学院,杨凌712100 [2]西安百跃羊乳集团有限公司,西安710000 [3]陕西省羊乳产品质量监督检验中心,富平711700

出　　处：《分析化学》2022年第2期278-289,共12页Chinese Journal of Analytical Chemistry

基　　金：校企合作项目(No.K4030220076)资助。

摘　　要：人乳寡糖(Human milk oligosaccharides,HMOs)与婴幼儿生命初期的生长发育密切相关,揭示其组成及结构有助于阐明HMOs功能。本研究通过超高效液相色谱-高分辨质谱联用技术,对19个HMOs(16个中性HMOs、3个酸性HMOs)进行了结构解析,在寡糖异构体裂解规律的基础上详细探讨了4组异构体的特征碎片离子,比较了3种超高效液相色谱条件下HMOs异构体分离效果的差异,为复杂HMOs异构体的结构解析及高效液相色谱条件的选择提供了科学依据。Human milk oligosaccharides(HMOs)are closely related to the growth and development of infants in early life.In this study,the structures of 19 HMOs(16 neutral HMOs and 3 sialylated HMOs)were analyzed by ultra-high performance liquid chromatography-high resolution mass spectrometry(UHPLC-HRMS).Based on the cleavage rules of oligosaccharide isomers,the differences of fragment ions among the four groups of isomers were discussed.The separation effects of HMOs isomers under three different UHPLC conditions were compared,which provided scientific basis for the structural analysis of complex HMOs isomers and the selection of UHPLC conditions.

关 键 词：人乳寡糖 超高效液相色谱-高分辨质谱 洗脱条件 同分异构体 结构解析 

分 类 号：O657.63[理学—分析化学] R341[理学—化学]