复方七芍降压片通过调控MMP-2/TIMP-2对冠心病大鼠血管重塑及动态血压的影响  

Effect of Compound Qishao Jiangya Tablet on Vascular Remodeling and Ambulatory Blood Pressure in Rats with Coronary Heart Disease by Regulating MMP-2/TIMP-2

作　　者：宣学习 张宇[1] 张华[1] 张传西 袁义强[1] XUAN Xuexi;ZHANG Yu;ZHANG Hua;ZHANG Chuanxi;YUAN Yiqiang(The Seventh People′s Hospital of Zhengzhou,Zhengzhou 450016,Henan,China)

机构地区：[1]郑州市第七人民医院,郑州450016

出　　处：《中西医结合心脑血管病杂志》2022年第2期229-234,共6页Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease

基　　金：河南省医学科技攻关计划项目(No.2017T02096)。

摘　　要：目的探讨复方七芍降压片通过调控基质金属蛋白酶-2(MMP-2)/组织金属蛋白酶抑制物-2(TIMP-2)对冠心病大鼠血管重塑的作用及对动态血压的影响。方法60只大鼠,随机取10只作为空白对照组(A组),另外50只采用冠状动脉结扎法制备冠心病大鼠模型,造模成功44只,随机分为模型组(B组)、卡托普利组(C组)、复方七芍降压片低剂量组(D组)、复方七芍降压片高剂量组(E组),各11只。采用无创血压仪检测各组大鼠收缩压、舒张压;酶联免疫吸附法(ELISA)检测白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、丙二醛(MDA)、活性氧(ROS);苏木精-伊红(HE)染色法观察胸主动脉形态学改变,检测血管重塑参数中膜厚度(MT)、管腔内径(LD),计算MT/LD;蛋白质印迹法(Western Blot)检测MMP-2、TIMP-2蛋白相对表达量。结果治疗1周、2周、3周、4周时,与A组比较,B组收缩压及舒张压升高(P<0.05);与B组比较,C组、D组、E组收缩压及舒张压降低(P<0.05);与D组比较,C组、E组收缩压及舒张压降低(P<0.05)。与A组比较,B组IL-1β、IL-6、TNF-α、ROS、MDA升高(P<0.05);与B组比较,C组、D组、E组IL-1β、IL-6、TNF-α、ROS、MDA降低(P<0.05);与D组比较,C组、E组IL-1β、IL-6、TNF-α、ROS、MDA降低(P<0.05)。HE染色显示,D组内皮增生程度减轻,部分平滑肌细胞向内膜下增生迁移,弹性纤维稍增多,C组、E组管壁无明显增厚,可见轻度内皮增生增厚,中膜平滑肌层、弹性纤维无明显病理改变。与A组比较,B组MT、MT/LD、MMP-2蛋白相对表达量及MMP-2/TIMP-2比值升高(P<0.05);与B组比较,C组、D组、E组MT、MT/LD、MMP-2蛋白相对表达量及MMP-2/TIMP-2比值降低(P<0.05);与D组比较,C组、E组MT、MT/LD、MMP-2蛋白相对表达量及MMP-2/TIMP-2降低(P<0.05);与A组比较,B组LD、TIMP-2蛋白相对表达量降低(P<0.05);与B组比较,C组、D组、E组LD、TIMP-2蛋白相对表达量升高(P<0.05);与D组比较,C组、E组Objective To investigate the effect of Compound Qishao Jiangya Tablet on vascular remodeling and ambulatory blood pressure in rats with coronary heart disease by regulating matrix metalloproteinase-2(MMP-2)/tissue inhibitor of metalloproteinase-2(TIMP-2).Methods Sixty rats were selected,and 10 rats were randomly selected as blank control group(group A).The remaining 50 rats were prepared as rat models with CHD by coronary artery ligation,and 44 rats were successfully modeled.The CHD modle rats were randomly divided into model group(group B),captopril group(group C),low-dose Compound Qishao Jiangya Tablet group(group D),and high-dose Compound Qishao Jiangya Tablet group(group E),with 11 rats in each group.The systolic blood pressure and diastolic blood pressure were measured by noninvasive blood pressure meter.The levels of interleukin-1β(IL-1β),interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),malondialdehyde(MDA),and reactive oxygen species(ROS)were detected by enzyme-linked immunosorbent assay(ELISA).The morphological changes of thoracic aorta were observed by hematoxylin eosin(HE)staining.The medial thickness(MT)and luminal diameter(LD)of vascular remodeling parameters were detected.The expressions of MMP-2 and TIMP-2 were detected by Western Blot.Results At 1,2,3,and 4 weeks of treatment,compared with group A,systolic blood pressure and diastolic blood pressure in group B increased(P<0.05).Compared with group B,systolic blood pressure and diastolic blood pressure of group C,group D,and group E decreased(P<0.05).Compared with group D,systolic blood pressure and diastolic blood pressure in group C and group E decreased(P<0.05).Compared with group A,IL-1β,IL-6,TNF-α,ROS,and MDA in group B increased(P<0.05).Compared with group B,IL-1β,IL-6,TNF-α,ROS,and MDA in group C,group D,and group E decreased(P<0.05).Compared with group D,IL-1β,IL-6,TNF-α,ROS and MDA in group C and group E decreased(P<0.05).HE staining showed that,the degree of endothelial proliferation was reduced,some smooth muscle cells migrate

关 键 词：冠心病 复方七芍降压片 基质金属蛋白酶-2 组织金属蛋白酶抑制物-2 血管重塑 动态血压 实验研究 

分 类 号：R73[医药卫生—肿瘤]