不同分化程度的肝细胞癌患者血清可溶性MICA水平及MICA等位基因多态性分析  

Analysis of serum sMICA levels and MICA polymorphisms in patients with hepatocellular carcinoma with different degrees of differentiation

作　　者：罗伟光 黄宏君[2] 罗奇志 Luo Weiguang;Huang Hongjun;Luo Qizhi(Department of Laboratory Medicine,Henan Provincial People’s Hospital;People’s Hospital of Zhengzhou University,Zhengzhou,Henan 450000,China)

机构地区：[1]河南省人民医院检验科,郑州大学人民医院,河南郑州450000 [2]湖南省肿瘤医院检验科,湖南长沙410013 [3]中南大学基础医学院免疫学系,湖南长沙410008

出　　处：《湘南学院学报（医学版）》2021年第4期1-7,共7页Journal of Xiangnan University（Medical Sciences）

基　　金：湖南省自然科学基金项目(2020JJ4768)。

摘　　要：目的研究肝细胞癌(hepatocellular carcinoma,HCC)患者血清可溶性MICA(soluble MHC class-Ⅰchain related gene A,sMICA)水平及MICA等位基因多态性与HCC分化程度的关系,为HCC的预防和治疗提供新的思路。方法按照Edmondson-Steiner四级(Ⅰ-Ⅳ)分级法将113例HCC患者分为高分化(Ⅰ-Ⅱ级)组(n=74)和低分化(Ⅲ-Ⅳ级)组(n=39),同时招募80例无亲缘关系的健康志愿者作为正常对照。收集对照组和HCC患者的外周血,通过酶联免疫吸附法(ELISA)检测各组血清sMICA水平,采用聚合酶链反应-直接测序法(PCR-SBT)对各组MICA等位基因进行测序分型。结果HCC组血清ALT、AST、AFP、sMICA的浓度高于对照组(P<0.001)。HCC低分化组的血清sMICA浓度高于HCC高分化组(P=0.007),HCC高分化组和低分化组的血清ALT、AST、AFP浓度差异无统计学意义(P>0.05)。MICA-A4在HCC组的频率(11.5%)明显低于对照组(23.1%)(Pc=0.01),而MICA-A5在HCC组的频率(48.2%)则高于对照组(34.4%)(Pc=0.03),但MICA-A4、MICA-A5在HCC高分化组与低分化组的分布频率无明显差异(Pc值分别为0.67、0.31)。MICA*010在HCC组的频率(36.7%)明显高于对照组(15.6%)(Pc<0.001),而MICA*045在HCC组的频率(3.1%)低于对照组(13.1%)(Pc<0.001),但MICA*010、MICA*045在HCC高分化组与低分化组的分布频率差异无统计学意义(Pc值分别为0.28、0.42)。结论血清sMICA水平可作为HCC恶性程度的一种重要标志,MICA-A5和MICA*010可能是HCC的一个易感因素,MICA-A4和MICA*045可能是HCC的一个保护因素。Objective To explore the correlation between the MICA(MHC class-Ⅰchain related gene A)polymorphisms,the amount of soluble MICA(sMICA)in the serum and the degree of hepatocellular carcinoma(HCC)differentiation,providing a novel idea for prevention and treatment of HCC.Methods The Edmondson-Steiner grade(Ⅰ-Ⅳ)was used to classify 113 HCC patients into two groups:well differentiated(gradeⅠ-Ⅱ,n=74)and poorly differentiated(gradeⅢ-Ⅳ,n=39).80 unrelated healthy volunteers were recruited as the control group.The peripheral blood of HCC groups and the control group were collected.The concentration of serum sMICA was detected by enzyme-linked immunosorbent assay(ELISA)and the polymorphism of MICA was genotyped by polymerase chain reaction-direct sequencing(PCR-SBT).Results The concentration of ALT、AST、AFP、serum sMICA in the HCC groups was higher than that in the control group(P<0.001).The concentration of sMICA in the HCC poorly differentiated group was higher than that in the HCC well differentiated group(P=0.007).There was no significant difference in the levels of ALT,AST,and AFP between the HCC well differentiated group and HCC poorly differentiated group(P>0.05).The frequency of MICA-A4 in the HCC groups(11.5%)was significantly lower than that in the control group(23.1%,Pc=0.01),while the frequency of MICA-A5 in the HCC group(48.2%)was higher than that in the control group(34.4%,Pc=0.03).However,micA-A4 and MICA-A5 showed no significant difference in the distribution frequency between the HCC well differentiated group and the HCC poorly differentiated group(Pc value was 0.67 and 0.31,respectively).The frequency of MICA*010 in HCC groups(36.7%)was significantly higher than that in the control group(15.6%;Pc<0.001),while the frequency of MICA*045 in HCC group(3.1%)was lower than that in control group(13.1%;Pc<0.001).However,there was no significant difference in the distribution frequency of MICA*010 and MICA*045 in the well differentiated and poorly differentiated HCC groups(Pc value was 0.28 and 0.4

关 键 词：MHCⅠ类链相关基因A 基因多态性 肝细胞癌 等位基因 分化 

分 类 号：R735.7[医药卫生—肿瘤]