HBV相关肝脏纤维化患者血清miRNA差异表达谱的建立及分析  

Establishment and analysis of serum miRNA differential expression profile in patients with HBV-associated liver fibrosis

作　　者：王卫敏[1] 李长安[1] 郑辉[2] 狄贯腾 WANG Weimin;LI Changan;ZHENG Hui;DI Guanteng(Department of Infectious Diseases,the Third Affiliated Hospital of Xinxiang Medical University,Xinxiang 453000,Henan,China;Department of Clinical Laboratory,the Third Affiliated Hospital of Xinxiang Medical University,Xinxiang 453000,Henan,China;Department of Public Health,the Third Affiliated Hospital of Xinxiang Medical University,Xinxiang 453000,Henan,China)

机构地区：[1]新乡医学院第三附属医院感染科,河南新乡453000 [2]新乡医学院第三附属医院检验科,河南新乡453000 [3]新乡医学院第三附属医院公共卫生科,河南新乡453000

出　　处：《临床检验杂志》2021年第12期892-896,共5页Chinese Journal of Clinical Laboratory Science

基　　金：国家十三五科技重大专项课题(2017ZX10203202-001);国家科技重大专项课题(2013ZX10002005)。

摘　　要：目的筛选HBV相关肝脏纤维化患者血清中差异表达的miRNA,并分析该类差异表达的miRNA在HBV相关肝纤维化进展过程中的作用。方法选择2019年3月至2020年3月于新乡医学院第三附属医院确诊为肝纤维化患者70例(包括乙肝患者35例,非乙肝患者35例),另收集35例体检健康者作为健康人对照组。收集各研究对象年龄、性别、血清学指标等临床资料。深度测序分析血清miRNA的表达水平;qRT-PCR验证候选表达差异显著的miRNA在各组中的表达水平。通过miRror 2.0数据库预测miRNA的靶基因,采用DAVID v6.7软件进行GO分析。结果与健康人对照组和非乙肝组相比,乙肝组中miR-505-3p(P=2.75×10^(-2))、miR-197-3p(P=4.57×10^(-2))和miR-500a-3p(P=1.28×10^(-3))的表达水平显著下调,而miR-33a-5p的表达水平显著上调(P=5.92×10^(-3))。GO分析结果表明,上述差异表达的miRNA所对应的靶基因主要涉及的生物学过程包括细胞的生长、维持和信号转导。结论HBV相关肝纤维化患者血清中miRNA的表达具有特异性,且与HBV相关肝纤维化过程关系密切。Objective To analyze and screen differentially expressed miRNAs in serum of patients with HBV-associated liver fibrosis.Methods A total of 70 patients with liver fibrosis diagnosed in our hospital from March 2019 to March 2020 were selected,including 35 patients with hepatitis B and 35 patients without hepatitis B.35 healthy subjects were selected as controls.The clinical data including age,gender and serological indexes of the subjects were collected.The expression of miRNA in serum was analyzed by Illumina miRNA deep sequencing.R platform was used for hierarchical cluster analysis.The expression of candidate miRNAs with significantly different expressions in each group was verified by qRT-PCR.The target genes of miRNA were predicted through the Mirror 2.0 database,and GO analysis was performed using David v6.7 software.Results Compared with the healthy group and the non-hepatitis B group,miR-505-3p(P=2.75×10^(-2)),miR-197-3p(P=4.57×10^(-2))and miR-500a-3p(P=1.28×10^(-3))in the hepatitis B group were significantly down-regulated,miR-33a-5p(P=5.92×10^(-3))was significantly up-regulated.Conclusion The expression of miRNA in serum of patients with HBV-associated liver fibrosis is specific,and these differentially expressed miRNAs may play an important role in the progression of HBV-associated liver fibrosis.

关 键 词：肝纤维化微小RNA 差异表达 生物学标志物 

分 类 号：R575.1[医药卫生—消化系统]