食管癌组织中肿瘤浸润性树突状细胞表型改变及功能分析  

Phenotypic changes and functional analysis of tumor infiltrating dendritic cells in esophageal cancer tissues

作　　者：王坤 吕胜祥 WANG Kun;LYU Shengxiang(Department of Gastroenterology,Lianyungang First People's Hospital,Jiangsu Lianyungang 222061,China)

机构地区：[1]连云港市第一人民医院消化内科,江苏连云港222061

出　　处：《现代肿瘤医学》2022年第2期253-257,共5页Journal of Modern Oncology

摘　　要：目的:探讨食管癌患者肿瘤组织中肿瘤浸润性树突状细胞表型改变及功能。方法:收集2017年01月至2018年09月我院收治的食管癌患者92例作为观察组,选择同时期我院收治的92例食管良性肿物患者作为对照组。术中分别取食管癌标本和良性肿物标本。检测相应组织中肿瘤浸润性树突状细胞表达水平及表型,同时检测T细胞亚群表达情况,分析肿瘤浸润性树突状细胞表达情况和食管癌患者临床特征的相关性。结果:与对照组比较,观察组肿瘤浸润性树突状细胞密度、MHC-Ⅱ阳性树突状细胞和CD54阳性树突状细胞百分比均显著降低(P<0.05);观察组CD4^(+)T细胞增高[(24.81±3.72)%vs(20.77±3.63)%,P=0.000];CD8^(+)T细胞降低[(20.90±4.12)%vs(23.08±4.42)%,P=0.001]。食管癌组织中肿瘤浸润性树突状细胞密度、MHC-Ⅱ阳性树突状细胞和CD54阳性树突状细胞百分比与肿瘤直径、TNM分期和淋巴结转移有关(P<0.05)。食管癌组织中肿瘤浸润性树突状细胞密度、MHC-Ⅱ阳性树突状细胞和CD54阳性树突状细胞与CD4^(+)T细胞显著负相关,与CD8^(+)T细胞显著正相关(P<0.05)。结论:肿瘤浸润性树突状细胞在食管癌组织中低表达,功能低下,与T细胞亚群失衡和预后不良有关。Objective:To investigate the phenotype and function of tumor infiltrating dendritic cells in esophageal cancer.Methods:From January 2017 to September 2018,92 patients with esophageal cancer admitted to our hospital were prospectively collected as the observation group,and 92 patients with benign esophageal masses admitted to our hospital during the same period were selected as the control group.Intraoperative samples of esophageal cancer,adjacent tissues and benign masses were taken.The expression level and phenotype of tumor infiltrating dendritic cells in the above tissues were detected.The expression of T cell subsets was detected as well.The correlations between tumor infiltrating dendritic cells and clinical characteristics of esophageal cancer patients were studied.Results:When compared with the control group,the density of tumor infiltrating dendritic cells,the percentage of MHC-II positive dendritic cells and CD54 positive dendritic cells in the observation group were significantly decreased(P<0.05).The CD4^(+)T cells in the observation group were increased[(24.81±3.72)%vs(20.77±3.63)%,P=0.000].CD8^(+)T cells in the observation group were decreased[(20.90±4.12)%vs(23.08±4.42)%,P=0.001].The density of tumor infiltrating dendritic cells,the percentage of MHC-II positive dendritic cells and CD54 positive dendritic cells in esophageal cancer were correlated with tumor diameter,TNM stage and lymph node metastasis(P<0.05).The density of tumor infiltrating dendritic cells,MHC-II positive dendritic cells and CD54 positive dendritic cells were negatively correlated with CD4^(+)T cells and positively correlated with CD8^(+)T cells in esophageal cancer tissues(P<0.05).Conclusion:Tumor infiltrating dendritic fineness is low expression and incompetent in esophageal cancer,which is related to imbalance of T cell subsets and poor prognosis.

关 键 词：食管癌 肿瘤浸润性树突状细胞 T细胞亚群 表型 

分 类 号：R735.1[医药卫生—肿瘤]