生物信息学分析FERMT1在膀胱癌组织中的表达及意义  

FERMT1 expression and significance in bladder carcinoma tissues by bioinformatics analysis

作　　者：郭小会 李策[1,2,3] 宫丽宝 李亚天 车晓芳 曲秀娟[1,2,3] GUO Xiaohui;LI Ce;GONG Libao;LI Yatian;CHE Xiaofang;QU Xiujuan(Department of Medical Oncology,the First Hospital of China Medical University,Liaoning Shenyang 110001;Key Laboratory of Anticancer Drugs and Biotherapy of Liaoning Province,the First Hospital of China Medical University,Liaoning Shenyang 110001;Liaoning Province Clinical Research Center for Cancer,Liaoning Shenyang 110001,China)

机构地区：[1]中国医科大学附属第一医院肿瘤内科,辽宁沈阳110001 [2]中国医科大学附属第一医院辽宁省抗癌药物与生物治疗重点实验室,辽宁沈阳110001 [3]辽宁省肿瘤研究中心,辽宁沈阳110001

出　　处：《现代肿瘤医学》2022年第1期71-76,共6页Journal of Modern Oncology

基　　金：“重大新药创制”科技重大专项(编号:2017ZX09304025);辽宁省重点研发指导计划资源平台建设项目(编号:2018225060)。

摘　　要：目的:生物信息学分析FERMT1在膀胱癌组织中的表达水平及其与病理参数的关系,并进而挖掘其突变信息及调控网络。方法:首先利用GEPIA数据库分析FERMT1在多种癌症中的表达水平,进而利用Oncomine及THPA等在线网站分析膀胱癌组织中FERMT1基因的mRNA及蛋白质表达情况;利用在线UALCAN数据库展现FERMT1与临床病理参数间的关系;cBioPortal在线数据库分析FERMT1基因的突变情况;LinkedOmics软件探究与FERMT1共表达的基因及基因富集分析。结果:FERMT1在多数癌症中呈现高表达,在膀胱癌组织中mRNA及蛋白水平均发生明显上调,且FERMT1表达水平与膀胱癌临床病理参数密切相关;同时,FERMT1基因发生多种类型的拷贝数突变,其中错义突变最为常见;FERMT1与多种基因存在共表达关系,进一步的富集分析展示这些基因在多种生物学过程中发挥重要作用。结论:本研究有效地揭示了FERMT1在膀胱癌组织中的表达信息及潜在的调控网络,为进一步研究FERMT1在膀胱癌组织发生发展中的作用奠定了理论基础。Objective:To analyze the expression level of FERMT1 in bladder cancer and its relationship with pathological parameters by bioinformatics,and to mine its mutation information and regulatory network.Methods:Firstly,the GEPIA database was used to analyze the expression level of FERMT1 in multiple cancers,and then online websites such as Oncomine and THPA were used to analyze the mRNA and protein expression of FERMT1 gene in bladder cancer.The online UALCAN database was used to show the relationship between FERMT1 and clinicopathological parameters relationship.cBioPortal online database analyzed the mutation of FERMT1 gene.LinkedOmics software explored the genes co-expressed with FERMT1 and gene enrichment analysis.Results:FERMT1 was highly expressed in most cancers,for bladder cancer,FERMT1 mRNA and protein levels were significantly up-regulated which was closely related to the clinicopathological parameters.At the same time,several types of copy number mutations occurred in FERMT1 gene,and missense mutations were the most common.FERMT1 co-expressed with a variety of genes,and further enrichment analysis shows that these genes played an important role in a variety of biological processes.Conclusion:This study effectively revealed the expression information and potential regulatory network of FERMT1 in bladder cancer,laying a theoretical foundation for further research on the role of FERMT1 in the development of bladder cancer.

关 键 词：膀胱癌 FERMT1 生物信息学分析 预后 

分 类 号：R737.14[医药卫生—肿瘤]