CCND1在人骨肉瘤组织中的表达分析  

Expression of CCND1 in human osteosarcoma tissue

作　　者：蔡承魁 田丽颖 马琼 马保安 CAI Chengkui;TIAN Liying;MA Qiong;MA Baoan(Department of Orthopedics,the Second Affiliated Hospital of Shaanxi University of Chinese Medicine,Shaanxi Xianyang 712000,China;Department of Anesthesiology,the Second Affiliated Hospital of Air Force Medical University,Shaanxi Xi'an 710038,China;Department of Orthopedics,the Second Affiliated Hospital of Air Force Medical University,Shaanxi Xi' an 710038,China)

机构地区：[1]陕西中医药大学第二附属医院骨伤一科,陕西咸阳712000 [2]空军军医大学第二附属医院麻醉科,陕西西安710038 [3]空军军医大学第二附属医院骨科,陕西西安710038

出　　处：《现代肿瘤医学》2022年第4期566-569,共4页Journal of Modern Oncology

基　　金：国家自然科学基金项目(编号:81201633)。

摘　　要：目的:通过检测骨肉瘤组织中细胞周期蛋白D1(cyclin D1,CCND1)基因的表达改变情况,明确骨肉瘤中CCND1基因的变化,探索CCND1基因在骨肉瘤发生和发展过程中的作用。方法:使用免疫组织化学法检测骨肉瘤组织和瘤旁组织中的CCND1表达差异,再利用实时定量PCR法对同种样本进行CCND1基因mRNA表达的相对定量测定,对比两种实验结果的异同。结果:免疫组织化学实验结果表明,在骨肉瘤组织中,CCND1表达较瘤旁组织增高,差异有统计学意义(P<0.05)。实时定量PCR实验结果进一步表明,CCND1基因mRNA在骨肉瘤组织中的表达是瘤旁组织的2.55倍,差异有统计学意义(P<0.05)。结论:两种实验结果表明,在骨肉瘤组织中CCND1表达量增多,意味着CCND1基因在骨肉瘤组织内处于高度活跃状态。Objective:To investigate the role of cyclin D1(CCND1)in the occurrence and development of osteosarcoma by detecting the change of the expression of the CCND1 in osteosarcoma tissue.Methods:The difference of CCND1 expression between osteosarcoma tissue and peritumoral tissue was detected by immunohistochemistry,andthe mRNA expression of CCND1 was detected by real-time quantitative PCR,and the similarities and differences between the two experimental results were compared.Results:The result of immunohistochemistry experiment showed that the expression of CCND1 in osteosarcoma tissue was higher than that in the peritumoral tissue,and the difference was statistically significant(P<0.05).Real-time quantitative PCR result further showed that the mRNA expression of CCND1 in osteosarcoma tissue was 2.55 times higher than that in the peritumoral tissue,and the difference was statistically significant(P<0.05).Conclusion:The results of the two experiments showed that the expression of CCND1 increased in osteosarcoma tissue,which indicate that CCND1 was up-regulated in osteosarcoma tissue.

关 键 词：骨肉瘤 细胞周期蛋白D1 免疫组织化学 实时定量PCR 

分 类 号：R738.1[医药卫生—肿瘤]