miR-409-3p调控靶基因Beclin-1抑制小细胞肺癌细胞的自噬进而抑制其生长和转移  

miR-409-3p regulates the target gene Beclin-1 to inhibit the autophagy of small cell lung cancer cells to inhibit its growth and metastasis

作　　者：韩忠诚[1] 王凤霞 马丽丽[1] 柳江[1] HAN Zhongcheng;WANG Fengxia;MA Lili;LIU Jiang(Department of Oncology,People's Hospital of Xinjiang Uygur Autonomous Region,Xinjiang Urumqi 830000,China;Department of Oncology,Yili Hospital of Traditional Chinese Medicine,Xinjiang Yili 835000,China)

机构地区：[1]新疆维吾尔自治区人民医院肿瘤科,新疆乌鲁木齐830000 [2]伊犁州中医医院肿瘤科,新疆伊犁835000

出　　处：《现代肿瘤医学》2022年第4期570-575,共6页Journal of Modern Oncology

基　　金：新疆维吾尔自治区自然科学基金项目(编号:2019D01C100);吴阶平医学基金会临床科研专项资助基金(编号:320.6750.2021-02-12)。

摘　　要：目的:探究miR-409-3p及其靶基因对小细胞肺癌细胞自噬、生长和转移的影响。方法:qRT-PCR检测miR-409-3p在HPAEpiC、SBC-5、NCI-H446、HOP-92细胞中的表达,SBC-5细胞中转染miR-409-3p mimic和miR-NC,CCK8法检测转染后细胞增殖,Transwell小室法检测细胞迁移和侵袭,流式细胞术检测细胞凋亡,双荧光素酶报告基因实验检测miR-409-3p和Beclin-1的靶向结合,SBC-5细胞中转染miR-409-3p mimic后转染Beclin-1过表达质粒(miR-409-3p mimic+Beclin-1),然后检测各组细胞增殖、迁移、侵袭和细胞凋亡水平。Western blot检测各组细胞中Beclin-1、LC3-Ⅱ、p62的表达。结果:miR-409-3p在SBC-5、NCI-H446、HOP-92细胞中的表达显著低于HPAEpiC细胞。miR-409-3p mimic组细胞增殖、迁移、侵袭能力显著低于miR-NC组,凋亡水平显著高于miR-NC组。双荧光素酶报告基因实验检测结果显示miR-409-3p靶向结合Beclin-1。miR-409-3p mimic+Beclin-1组细胞增殖、迁移、侵袭能力显著高于miR-409-3p mimic组,细胞凋亡水平显著低于miR-409-3p mimic组。miR-409-3p mimic组细胞Beclin-1、LC3-Ⅱ表达显著低于miR-NC组,p62表达显著高于miR-NC组。miR-409-3p mimic+Beclin-1组细胞LC3-Ⅱ表达显著高于miR-409-3p mimic组,p62表达显著低于miR-409-3p mimic组。结论:miR-409-3p调控靶基因Beclin-1抑制SBC-5细胞生长和转移,其机制可能为抑制小细胞肺癌细胞的自噬。Objective:To explore the effects of miR-409-3p and its target gene on autophagy,growth and metastasis of small cell lung cancer cells.Methods:qRT-PCR was used to detect the expression of miR-409-3p in HPAEpiC,SBC-5,NCI-H446,HOP-92 cells.miR-409-3p mimic and miR-NC were transfected into SBC-5 cells,and the CCK8 method was used to detect cell proliferation.Transwell chamber method was used to detect cell migration and invasion.Flow cytometry was used to detect cell apoptosis.Dual luciferase reporter gene experiment was used to detect the targeted binding of miR-409-3p and Beclin-1.After transfecting with miR-409-3p mimic,the Beclin-1 overexpression plasmid(miR-409-3p mimic+Beclin-1)was transfected to SBC-5 cells,and then the levels of cell proliferation,migration,invasion and apoptosis of each group were detected.Western blot was used to detect the expression of Beclin-1,LC3-Ⅱ,and p62 in each group of cells.Results:The expression of miR-409-3p in SBC-5,NCI-H446,and HOP-92 cells was significantly lower than that in HPAEpiC cells.The cell proliferation,migration and invasion ability in miR-409-3p mimic group was significantly lower than that in miR-NC group,and the level of apoptosis was significantly higher than that in miR-NC group.The test results of dual luciferase reporter gene experiment showed that miR-409-3p targeted Beclin-1.The cell proliferation,migration and invasion ability of miR-409-3p mimic+Beclin-1 group was significantly higher than that in miR-409-3p mimic group,and the level of cell apoptosis was significantly lower than that in miR-409-3p mimic group.Beclin-1 expression and LC3-Ⅱexpression in miR-409-3p mimic group were significantly lower than miR-NC group,and p62 expression was significantly higher than miR-NC group.The LC3-Ⅱexpression of the miR-409-3p mimic+Beclin-1 group was significantly higher than that in the miR-409-3p mimic group,and the p62 expression was significantly lower than that in the miR-409-3p mimic group.Conclusion:miR-409-3p regulates the target gene Beclin-1 to inhibi

关 键 词：miR-409-3p BECLIN-1 小细胞肺癌 生长和转移 

分 类 号：R734.2[医药卫生—肿瘤]