BIRC5基因在非小细胞肺癌中的表达及与免疫细胞浸润和预后的关系  

BIRC5 gene expression in non-small cell lung cancer and its correlation with immune cells infiltration and prognosis

作　　者：郑浩然 蒋爱民 史楚楚 傅潇 姚煜[1] ZHENG Haoran;JIANG Aimin;SHI Chuchu;FU Xiao;YAO Yu(Department of Medical Oncology,the First Affiliated Hospital of Xi'an Jiaotong University,Shaanxi Xi'an 710061,China)

机构地区：[1]西安交通大学第一附属医院肿瘤内科,陕西西安710061

出　　处：《现代肿瘤医学》2022年第4期634-640,共7页Journal of Modern Oncology

基　　金：陕西省自然科学基础研究计划项目(编号:2020JQ-512);陕西省重点研发计划项目(编号:2019KW-074)。

摘　　要：目的:通过生物信息学方法分析杆状病毒凋亡抑制蛋白5(baculoviral inhibitor of apoptosis repeat-containing protein 5,BIRC5)基因在非小细胞肺癌(non-small cell lung cancer,NSCLC)与正常组织中的表达差异,并分析其与NSCLC预后和免疫细胞浸润水平的关系。方法:运用GEPIA数据库分析NSCLC和正常肺组织中BIRC5基因的表达水平。运用Sperman's相关性检验在TIMER数据库和GEPIA数据库对BIRC5基因表达与NSCLC中免疫细胞浸润水平、免疫细胞浸润表面标记物的相关性进行分析。最后,通过Kaplan-Meier Plotter分析BIRC5表达水平与NSCLC预后的关系。结果:与正常组织相比,BIRC5基因在NSCLC组织中表达水平升高(P<0.05)。BIRC5基因与NSCLC免疫微环境中多种免疫细胞浸润水平及其表面标记物显著相关。多因素COX回归模型显示,B细胞浸润水平降低及BIRC5高表达是肺腺癌患者不良预后的独立危险因素(P<0.05)。生存分析结果表明BIRC5基因高表达与NSCLC患者的不良预后显著相关(P<0.05)。结论:BIRC5基因在NSCLC中表达上调,影响NSCLC免疫细胞浸润水平,导致免疫微环境失衡,进而造成不良的临床预后。BIRC5基因有望成为NSCLC免疫细胞浸润和预后相关的生物学标志物。Objective:To analyze the differences between the expression of baculoviral inhibitor of apoptosis repeat-containing protein 5(BIRC5)in non-small cell lung cancer(NSCLC)and normal tissues using bioinformatics analysis,and to analyze its relationship with NSCLC prognosis and immune cells infiltration level.Methods:The GEPIA database was utilized to investigate the expression level of BIRC5 in NSCLC and normal lung tissue.The correlation between BIRC5 expression and immune cells infiltration level and immune cells infiltration-related gene markers in NSCLC was analyzed by Sperman's correlation test on TIMER database and GEPIA database.Finally,the relationship between the expression of BIRC5 and NSCLC prognosis was analyzed by Kaplan-Meier plotter.Results:BIRC5 expression level in NSCLC was increased compared with normal tissues(P<0.05).BIRC5 gene is significantly related to the infiltration level of various immune cells and their genetic markers in NSCLC immuno-microenvironment.Multivariate Cox regression analysis showed that low infiltration of B cells and high expression of BIRC5 were independent risk factors of poor prognosis in lung adenocarcinoma(P<0.05).Survival analysis showed that high expression of BIRC5 was significantly associated with poor prognosis in NSCLC(P<0.05).Conclusion:The expression of BIRC5 gene was increased in NSCLC,which affected the level of NSCLC immune cells infiltration and resulting in the imbalance of immune microenvironment,which in turn caused poor clinical prognosis.BIRC5 gene is expected to be a biological marker associated with NSCLC immune cells infiltration and prognosis.

关 键 词：杆状病毒凋亡抑制蛋白5 非小细胞肺癌 预后 免疫浸润水平 

分 类 号：R734.2[医药卫生—肿瘤]