异体NK细胞治疗消化道恶性腹水的安全性与疗效(附3例)  

Safety and efficacy of allogeneic NK cells in the treatment of metastatic gastrointestinal malignant ascites(3 cases)

作　　者：张可 方珏敏[1] 杨娟 刘根桃 李济宇[1] 许青[1] ZHANG Ke;FANG Juemin;YANG Juan;LIU Gentao;LI Jiyu;XU Qing(Department of Oncology,Shanghai Tenth People's Hospital Affiliated to Tongji University,Shanghai 200072,China;Shanghai Biomed-union Biotechnology Co.Ltd,Shanghai 201321,China)

机构地区：[1]同济大学附属第十人民医院肿瘤科,上海200072 [2]上海科医联创生物科技有限公司,上海201321

出　　处：《现代肿瘤医学》2022年第4期646-651,共6页Journal of Modern Oncology

基　　金：上海市优秀学科带头人项目(编号:18XD1424000);上海卫生计生委重点项目(编号:201640020);上海市科委西医引导项目(编号:17411967300)。

摘　　要：目的:分析3例异体自然杀伤细胞(natural killer cells,NK)治疗转移性消化道肿瘤所致癌性腹水患者的安全性与临床有效性。方法:回顾性分析2018年我院肿瘤科进行的3例利用异体NK细胞治疗转移性消化道肿瘤所致癌性腹水患者。分析治疗前后患者腹水情况、外周血中各种细胞因子(IL-6、IL-8、IL-10、TNF-α)、肿瘤标志物(CEA、CA199)、淋巴细胞亚群、脱落癌细胞(exfoliated cancer cells,ECC)的变化以及患者腹水症状改善情况,观察其安全性与疗效。结果:转移性消化道肿瘤所致癌性腹水患者接受异体NK治疗后无严重不良反应。3例患者IL-8在异体NK细胞治疗后均有明显下降。1例患者在异体NK细胞治疗后腹水深度、腹水ECC、外周血CEA及CA199均有明显下降。但3例患者体内淋巴细胞亚群均无显著变化。结论:3例异体NK细胞治疗转移性消化道肿瘤所致癌性腹水患者安全无严重副作用。1例患者异体NK细胞治疗可能具有一定疗效。消化道恶性腹水异体NK细胞治疗的临床疗效有待进一步验证。Objective:To evaluate the safety and clinical efficacy of 3 patients with malignant ascites caused by metastatic gastrointestinal tumors treated with allogenetic NK cells.Methods:We collected the clinical data of three patients with malignant ascites caused by metastatic gastrointestinal carcinoma treated with allogenetic NK cells in our hospital.The changes in IL-6,IL-8,IL-10,TNF-α,lymphocyte subsets,tumor markers in peripheral blood of patients,ECC in ascites and patients'improvements of ascites symptoms were observed at pre-treatment and 2 weeks post-treatment.Adverse events of patients were also evaluated.Results:No serious infusion-related side effects were observed in all 3 patients.The IL-8 of 3 patients decreased significantly after allogeneic NK cell treatment.The depth of ascites,ascites ECC,peripheral blood CEA and CA199 decreased significantly in 1 patient after allogeneic NK cell treatment.No significant change was observed in lymphocyte subsets in 3 patients.Conclusion:Three patients with malignant ascites caused by metastatic digestive tract tumors treated with allogeneic NK cells were safe and without serious side effects.Allogeneic NK cell therapy may be effective in 1 patient.The clinical efficacy of allogeneic NK cell therapy needs to be further verified.

关 键 词：NK细胞 转移性消化道肿瘤 恶性腹水 细胞免疫疗法 

分 类 号：R730.51[医药卫生—肿瘤]