GRK6在胆管癌组织中的表达及对胆管癌细胞增殖和侵袭的影响  

Expression of GRK6 in cholangiocarcinoma tissues and its effect on proliferation and invasion of cholangiocarcinoma cells

作　　者：谭敏 薛栋 武学福 佘军军[1] 孙祺 TAN Min;XUE Dong;WU Xuefu;SHE Junjun;SUN Qi(Department of General Surgery,the First Affiliated Hospital of Xi'an Jiaotong University,Shaanxi Xi'an 710061,China)

机构地区：[1]西安交通大学第一附属医院普通外科,陕西西安710061

出　　处：《现代肿瘤医学》2022年第4期659-663,共5页Journal of Modern Oncology

基　　金：国家自然科学基金资助项目(编号:81702362)。

摘　　要：目的:检测GRK6在胆管癌组织及相应癌旁正常胆管上皮组织内的表达情况,并探究抑制GRK6的表达对胆管癌细胞克隆增殖及侵袭的影响。方法:通过qRT-PCR及免疫组织化学染色检测GRK6在胆管癌及癌旁正常组织中的表达情况,并分析其与不同临床病理因素之间的关系。通过RNA干扰技术抑制QBC939细胞中GRK6的表达,检测siRNA在QBC939细胞中对GRK6的抑制效果;采用MTT、平板克隆形成实验检测抑制GRK6表达对胆管癌细胞克隆增殖的影响,Transwell实验检测抑制GRK6表达对细胞侵袭的影响。结果:qRT-PCR和免疫组化结果均提示GRK6在胆管癌组织高表达,GRK6的高表达与肿瘤大小及TNM分期密切相关。si-GRK6可有效沉默QBC939细胞中GRK6的表达;MTT实验及平板克隆形成实验结果表明抑制GRK6表达能够抑制胆管癌细胞QBC939的增殖和克隆形成过程;Transwell实验结果表明抑制GRK6表达后,胆管癌细胞的侵袭能力明显降低。结论:GRK6在胆管癌细胞中高表达,抑制GRK6的表达能够抑制胆管癌细胞的增殖和侵袭能力。Objective:To detect the expression of GRK6 in bile duct carcinoma tissues and adjacent normal bile duct epithelial tissues,and to explore the effects of inhibiting GRK6 expression on the proliferation and invasion of bile duct carcinoma cells.Methods:The expression of GRK6 in cholangiocarcinoma and adjacent normal tissues was detected by qRT-PCR,immunohistochemical staining,and the relationship between GRK6 expression and different clinicopathological factors was analyzed.GRK6 expression in QBC939 cells was inhibited by RNA interference technique,and the inhibitory effect of siRNA on GRK6 in QBC939 cells was detected.The effects of GRK6 inhibition on the proliferation of bile duct cancer cells were detected by MTT and plate clone formation assay,and the effects of GRK6 inhibition on cell invasion were detected by Transwell assay.Results:Both qRT-PCR and immunohistochemical results indicated that GRK6 was highly expressed in cholangiocarcinoma tissues.The high expression of GRK6 was closely related to tumor size and TNM stage.si-GRK6 effectively silenced the expression of GRK6 in QBC939 cells.The results of MTT assay and plate clonogenesis assay showed that inhibition of GRK6 expression could inhibit the proliferation and clonogenesis of bile duct carcinoma cells QBC939.Transwell assay results showed that inhibition of GRK6 expression significantly reduced the invasion ability of bile duct cancer cells.Conclusion:GRK6 is highly expressed in bile duct cancer cells.Inhibition of GRK6 expression can inhibit the proliferation,invasion of bile duct cancer cells.

关 键 词：胆管癌 GRK6 细胞增殖 细胞侵袭 

分 类 号：R735.8[医药卫生—肿瘤]