Glypican-6在胃肠道间质瘤组织中的表达及其通过ERK1/2通路促进细胞的增殖、迁移及侵袭  

The expression of Glypican-6 in gastrointestinal stromal tumor tissues and it promotes the proliferation,migration and invasion of cells through the ERK1/2 pathway

作　　者：龚庆豪 高平发 顾志相 蔡一亭 GONG Qinghao;GAO Pingfa;GU Zhixiang;CAI Yiting(Department of Gastrointestinal Surgery,Chongming Branch of Shanghai Xinhua Hospital,Shanghai 202150,China)

机构地区：[1]上海新华医院崇明分院胃肠外科,上海202150

出　　处：《现代肿瘤医学》2022年第3期382-388,共7页Journal of Modern Oncology

基　　金：上海市崇明区“可持续发展科技创新行动计划”项目(编号:CKY2020-13)。

摘　　要：目的:探究Glypican-6对胃肠道间质瘤的作用及其可能的作用机制。方法:收集胃肠道间质瘤组织及其癌旁组织27例,RT-PCR和Western blot检测组织中Glypican-6的表达。选取胃肠道间质瘤细胞系GIST-T1进行体外研究。RT-PCR实验检测细胞中Glypican-6 mRNA的表达;Western blot检测细胞中Glypican-6、E-cadherin、N-cadherin、p-ERK1/2和ERK1/2蛋白的表达;CCK-8检测细胞增殖;Transwell实验检测细胞迁移和侵袭。结果:与癌旁组织相比,癌组织中Glypican-6 mRNA和蛋白表达均显著升高(P<0.05)。与si-NC组相比,si-Glypican-6组细胞中Glypican-6 mRNA和蛋白表达均显著降低(P<0.05),细胞24 h、48 h和72 h的增殖能力、迁移细胞数、侵袭细胞数、N-cadherin蛋白和p-ERK1/2/ERK1/2比值显著降低(P<0.05),E-cadherin蛋白表达显著升高(P<0.05);与pcDNA3.1组相比,pcDNA3.1-Glypican-6组细胞中Glypican-6 mRNA和蛋白表达均显著升高(P<0.05),细胞24 h、48 h和72 h的增殖能力、迁移细胞数、侵袭细胞数、N-cadherin蛋白表达和p-ERK1/2/ERK1/2比值显著升高(P<0.05),E-cadherin蛋白表达显著降低(P<0.05);与pcDNA3.1-Glypican-6组相比,pcDNA3.1-Glypican-6+PD98059组细胞24 h、48 h和72 h的增殖能力显著降低(P<0.05),迁移细胞数、侵袭细胞数、N-cadherin蛋白表达和p-ERK1/2/ERK1/2比值显著降低(P<0.05),E-cadherin蛋白表达显著升高(P<0.05),Glypican-6蛋白表达差异无统计学意义(P>0.05)。结论:Glypican-6在胃肠道间质瘤组织中高表达,Glypican-6通过ERK1/2通路促进胃肠道间质瘤细胞增殖能力、迁移能力、侵袭能力和N-cadherin蛋白表达,抑制E-cadherin蛋白表达。Objective:To explore the effect of Glypican-6 on gastrointestinal stromal tumors and its possible mechanism.Methods:Twenty-seven cases of gastrointestinal stromal tumor tissues and adjacent tissues were collected,and the expression of Glypican-6 in the tissues was detected by RT-PCR and Western blot.The gastrointestinal stromal tumor cell line GIST-T1 was selected for vitro studies.RT-PCR experiment was used to detect cell Glypican-6 mRNA expression.Western blot was used to detect cell Glypican-6,E-cadherin,N-cadherin,p-ERK1/2 and ERK1/2 protein expression.CCK-8 was used to detect cell proliferation.Transwell test was used to detect cell migration and invasion.Results:Compared with adjacent tissues,mRNA and protein expression of Glypican-6 in cancer tissues were significantly increased(P<0.05).Compared with the si-NC group,the expression of Glypican-6 mRNA and protein in si-Glypican-6 cells were significantly reduced(P<0.05).The proliferation capacity of cells at 24 h,48 h and 72 h,number of migrating cells and number of invasion cells,the expression of N-cadherin protein and p-ERK1/2/ERK1/2 rate were significantly reduced(P<0.05),and the expression of E-cadherin protein was significantly increased(P<0.05).Compared with the pcDNA3.1 group,the expression of Glypican-6 mRNA and protein in the cells of pcDNA3.1-Glypican-6 group were significantly increased(P<0.05),and the proliferation ability at 24 h,48 h and 72 h,number of migrating cells,number of invasion cells,the expression of N-cadherin protein and p-ERK1/2/ERK1/2 rate were significantly increased(P<0.05),and the expression of E-cadherin protein was significantly decreased(P<0.05).Compared with the pcDNA3.1-Glypican-6 group,the pcDNA3.1-Glypican-6+PD98059 group had significantly lower proliferation ability at 24 h,48 h and 72 h(P<0.05),and the number of migrating cells,the number of invading cells and the protein expression of N-cadherin and p-ERK1/2/ERK1/2 rate were significantly reduced(P<0.05),protein expression of E-cadherin was significantly increased(P<

关 键 词：Glypican-6 胃肠道间质瘤 ERK1/2通路 

分 类 号：R735[医药卫生—肿瘤]