GOLM1在肺腺癌组织中的表达及对A549细胞增殖、迁移和侵袭能力的影响  被引量:2

The expression of GOLM1 in lung adenocarcinoma tissues and its effect on the cell proliferation,migration and invasion of A549 cells

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作  者:贾春丽[1] 张华[1] 路鹏霏 杨颖[1] 李莉[2] 胡军[2] 朱海鹏[2] JIA Chunli;ZHANG Hua;LU Pengfei;YANG Ying;LI Li;HU Jun;ZHU Haipeng(Cancer Center,the First Affiliated Hospital of Xinjiang Medical University,Xinjiang Urumqi 830000,China;Hematology Oncology Department,Karamay Central Hospital,Xinjiang Karamay 834000,China)

机构地区:[1]新疆医科大学第一附属医院肿瘤中心,新疆乌鲁木齐830000 [2]克拉玛依市中心医院血液肿瘤科,新疆克拉玛依834000

出  处:《现代肿瘤医学》2022年第3期412-415,共4页Journal of Modern Oncology

基  金:新疆维吾尔自治区自然科学基金资助项目(编号:2019D01A07)。

摘  要:目的:探讨高尔基磷蛋白2(Golgi phosphoprotein 2,GOLPH2,又称为GP73或GOLM1)在肺腺癌组织中的表达及对A549细胞增殖、迁移、侵袭能力的影响。方法:从GEPIA数据库分析肺腺癌组织中GOLM1 mRNA表达情况及与肺腺癌患者预后的相关性;利用慢病毒siRNA技术下调人肺腺癌A549细胞中GOLM1的表达,通过Real-time PCR验证沉默效果,CCK-8、细胞划痕实验及Transwell实验检测细胞的增殖、迁移、侵袭能力。结果:GOLM1 mRNA在肺腺癌组织中高表达(P<0.05);GOLM1 mRNA高表达的肺腺癌患者的总生存期明显低于GOLM1低表达患者(P<0.05);沉默GOLM1表达后A549细胞的增殖、迁移及侵袭能力显著被抑制(P<0.05)。结论:GOLM1在肺腺癌组织中高表达,沉默其表达可抑制肺腺癌细胞增殖、迁移和侵袭能力,可以作为潜在的评估肺腺癌患者预后的标志物和治疗靶标。Objective:To investigate the expression of Golgi phosphoprotein 2(GOLM1)in lung adenocarcinoma tissues and the effect on proliferation,migration and invasion of A549 cells.Methods:The expression of GOLM1 mRNA in lung adenocarcinoma tissues and its correlation with prognosis were analyzed from GEPIA database.Lentiviral siRNA technology was used to down regulate the expression of GOLM1 in human lung adenocarcinoma A549 cells.Real-time PCR was used to verify the silencing effect.CCK-8,cell scratch test and Transwell test were used to detect the cell proliferation,migration and invasion.Results:GOLM1 mRNA was highly expressed in lung adenocarcinoma(P<0.05).The overall survival time of patients with high GOLM1 mRNA expression was significantly lower than that of patients with low GOLM1 expression(P<0.05).The proliferation,migration and invasion of A549 cells were significantly decreased after GOLM1 knockdown(P<0.05).Conclusion:GOLM1 is highly expressed in lung adenocarcinoma,silencing its expression can inhibit cell proliferation,migration and invasion of lung adenocarcinoma cells.It can be used as a potential prognostic marker and therapeutic target for lung adenocarcinoma.

关 键 词:高尔基磷蛋白2 肺腺癌 细胞增殖 细胞迁移 细胞侵袭 

分 类 号:R734.2[医药卫生—肿瘤]

 

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