大豆根际踝节真菌WR1-4的溶磷特性研究  

Study on Phosphorus Solubilization Characteristics of Talaromyces sp.WR1-4 in Soybean(Glycine max)Rhizosphere

作　　者：任氢欣 王继华[1] 曾齐 张必弦[2] 刘秀林[2] REN Qing-Xin;WANG Ji-Hua;ZENG Qi;ZHANG Bi-Xian;LIU Xiu-Lin(School of Life Science and Technology,Harbin Normal University,Harbin 150025,China;Soybean Research Institute,Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China)

机构地区：[1]哈尔滨师范大学生命科学与技术学院,哈尔滨150025 [2]黑龙江省农业科学院大豆研究所,哈尔滨150086

出　　处：《农业生物技术学报》2022年第1期114-124,共11页Journal of Agricultural Biotechnology

基　　金：“水体污染控制与治理”国家重大专项(2017ZX07202-002-06)。

摘　　要：溶磷真菌可以将土壤中潜在的无效磷转化为有效磷,是缓解土壤缺磷现象、提高磷肥利用率的一种有效途径。本研究对前期从大豆(Glycine max)根际土壤中筛选得到的一株踝节真菌(Talaromyces sp.)WR1-4的溶磷特性和产酸作用进行研究,分别采用平板培养法和液体培养法测定菌株WR1-4的溶磷能力;利用钼锑抗比色法对菌株WR1-4在难溶性磷源、碳源、氮源条件下的溶磷特性进行测定;利用HPLC检测WR1-4产生的有机酸。结果显示,WR1-4对5种难溶性磷源(磷酸钙,磷酸锌,磷酸铝,磷矿粉和磷酸铁)的溶解能力存在差异,有效磷增量分别为693.07、613.47、260.14、137.84和386.33 mg/L;菌株WR1-4利用6种碳源的顺序分别为葡萄糖、蔗糖、麦芽糖、淀粉、乳糖和纤维素;与其他氮源相比,WR1-4在硫酸铵条件下溶解磷的能力最强,在硝酸钾条件下溶解磷的能力最弱;菌株WR1-4在培养过程中可产生草酸、乳酸、柠檬酸、苹果酸、乙酸、富马酸、酒石酸和马来酸共8种有机酸;Pearson分析显示,有效磷的增加量与有机酸含量之间为正相关;在菌株WR1-4的生长溶磷阶段,环境pH有所下降。上述研究结果显示,菌株WR1-4溶磷能力较强、生长代谢条件较广,在微生物肥料研发及农业生产中具有潜在的应用前景。Phosphorus solibilizating fungus can convert the potentially ineffective phosphorus into available phosphorus in the soil,which is an effective way to alleviate the phenomenon of soil phosphorus deficiency and increase the utilization rate of phosphorus fertilizer.The strain WR1-4(Talaromyces sp.)was screened from the rhizosphere soil of soybean(Glycine max)in preliminary work.In order to clarify its phosphorus solubility and acid production,the phosphate solubilizing ability of strain WR1-4 was measured by plating method and broth method in present study;the molybdenum antimony anti colorimetric method was used to detect the phosphorus solubilizing characteristics of strain WR1-4 under different conditions of insoluble phosphorus,carbon and nitrogen sources;HPLC was used to detect the organic acids produced by WR1-4.The results showed that WR1-4 had different ability to dissolve 5 insoluble phosphates(calcium phosphate,zinc phosphate,aluminum phosphate,phosphate rock powder,iron phosphate),and the effective phosphorus increments were 693.07,613.47,260.14,137.84 and 386.33 mg/L,respectively.Strain WR1-4 used 6 carbon sources in the order of glucose,sucrose,maltose,starch,lactose and cellulose.As to different nitrogen sources,strain WR1-4 had strongest phosphate solubilizing capacity under the condition of ammonium sulfate,and least phosphate solubilizing ability under the condition of potassium nitrate.Strain WR1-4 could produce 8 kinds of organic acids,including oxalic acid,lactic acid,citric acid,malic acid,acetic acid,fumaric acid,tartaric acid,and maleic acid.According to Pearson analysis,the correlation between the increase of available phosphorus and organic acid was positively correlated.At the same time,while strain WR1-4 was in the stage of phosphate solubilization,the pH of the environment decreased.Above results indicate that strain WR1-4 had a strong ability to dissolve phosphorus and a wide range of growth and metabolism conditions,which makes it has potential application prospects in microbial ferti

关 键 词：溶磷真菌 碳源 氮源 有机酸 

分 类 号：S154.3[农业科学—土壤学] S565.1[农业科学—农业基础科学]