NMR-based metabolomic responses of freshwater gastropod Bellamya aeruginosa to MC-producing and non MC-producing Microcystis aeruginosa  

作　　者：Wen YANG Yangfang YE Kaihong LU Zhongming ZHENG Jinyong ZHU 

机构地区：[1]School of Marine Science,Ningbo University,Ningbo 315800,China

出　　处：《Journal of Oceanology and Limnology》2022年第1期260-272,共13页海洋湖沼学报（英文）

基　　金：Supported by the National Natural Science Foundation of China(Nos.42077219,31302192);the Natural Science Foundation of Zhejiang Province(No.LQ20C190003);the Ningbo Municipal Natural Science Foundation(No.2019A610421);the Ningbo Public Welfare Technology Application Research Project(No.202002N3049);the Hangzhou Municipal Agriculture and Social Development Project(No.2020ZDSJ0697),the Fundamental Research Funds for the Provincial Universities of Zhejiang(No.SJLY2020011),and the K.C.Wong Magna Fund in Ningbo University。

摘　　要：Molluscan metabolomic analysis is essential for the understanding of the regulatory mechanism of aquatic invertebrate in response to hepatotoxic microcystins(MCs)stress.To understand the system responses of the gastropod to MC exposure,metabolomic alterations caused by two strains(MC-producing and non MC-producing)of Microcystis aeruginosa were characterized indiff erent biological matrices(hepatopancreas and muscle)of Bellamya aeruginosa(Gastropoda)using 1 H nuclear magnetic resonance(NMR)spectroscopy combined with MCs detections after exposure for 1,7,and 14 d.Although ELISA analysis showed that no MCs was detected in both tissues after non MC-producing M.aeruginosa exposure,MCs concentrations were increasing in the hepatopancreas(from 1.29±0.48μg/g to 3.17±0.11μg/g)and foot muscle(from 0.07±0.02μg/g to 0.21±0.08μg/g)after 14-d exposure of MC-producing M.aeruginosa.Meanwhile,we observed that MC induced signifi cant increase in creatine,a variety of amino acids(leucine,isoleucine,valine,threonine,alanine,methionine,glutamate,aspartate,and lysine),carboxylic acids(lactate,acetate,and D-3-hydroxybutyrate),and choline and its derivatives(phosphocholine and glycerophosphocholine)but decreased the energy substance(lipids,glucose,and glycogen)in the hepatopancreas.However,no signifi cant metabolite diff erences were observed in the muscle between MC-producing and non MC-producing cyanobacteria treated groups.These results suggest that MC exposure may cause hepatic energy expenditure accompanied with various metabolic disorders that involve lipid metabolism,protein catabolism,osmoregulation,glycolysis,glycogenolysis,and tricarboxylic acid(TCA)cycle.Moreover,metabolic perturbation was aggravated as the level of accumulated MCs raised over time in the MC-producing cyanobacteria treatment.These fi ndings indicated that MCs accumulation might lead to oxidative-stress-mediated damage of mitochondria functions.

关 键 词：Bellamya aeruginosa Microcystis aeruginosa METABOLOMIC nuclear magnetic resonance MICROCYSTIN 

分 类 号：Q958[生物学—动物学]