生物信息学分析CA3在乳腺癌组织中的表达和对预后的影响  

Bioinformatics analysis of CA3 expression in breast cancer tissues and its impact on prognosis

作　　者：张江涛 阮晖朝 吴向华[1] ZHANG Jiangtao;RUAN Huichao;WU Xianghua(Department of Gastrointestinal Glandular Surgery,The First Affiliated Hospital of Guangxi Medical University,Nanning 530021,P.R.China)

机构地区：[1]广西医科大学第一附属医院胃肠腺体外科,南宁530021

出　　处：《中国普外基础与临床杂志》2022年第1期51-58,共8页Chinese Journal of Bases and Clinics In General Surgery

基　　金：广西自然科学基金项目(项目编号:2017GXNSFAA198066)。

摘　　要：目的通过多种在线数据库分析碳酸酐酶3(carbonic anhydrase 3,CA3)在乳腺癌中的表达差异、预后潜力以及相关机制。方法使用GEPIA2.0和TIMER数据库资料分析CA3 mRNA在乳腺癌组织中的表达差异;使用Bc-GenExMinerv4.7数据库资料分析乳腺癌亚分类的CA3 mRNA的表达差异;使用Kaplan-Meier绘图仪、Bc-GenExMinerv4.7和PrognoScan数据库资料分析CA3 mRNA表达水平与乳腺癌患者预后的关系;使用LinkedOmics数据库资料分析CA3共表达基因及其生物学行为;使用TIMER数据库资料分析乳腺癌组织中CA3 mRNA的表达与免疫细胞浸润的关系。结果乳腺癌组织中CA3 mRNA表达水平低于正常乳腺组织(P<0.05),在ER阴性(P<0.05)、PR阴性(P<0.05)、HER2阴性(P<0.05)以及无淋巴转移者(P<0.05)其表达水平较高,且在高表达Ki67乳腺癌患者中CA3表达水平较低(P<0.05),与SBR和NPI分级也具有相关性(P<0.05);CA3 mRNA表达水平低的乳腺癌患者其总体生存率、无复发生存率和无病生存率均较低(P<0.05);筛选出的正相关前50个共表达基因有10个基因具有低风险比(P<0.05),筛选出的负相关前50个共表达基因中有11个基因具有高风险比(P<0.05);在乳腺癌组织中CA3 mRNA表达与CD4^(+)T细胞和CD8^(+)T细胞具有正相关关系(r_(s)=0.175,P<0.001;r_(s)=0.137,P<0.001),与T细胞衰竭标志物LAG3、TIM-3和PVRL2呈负相关关系(r_(s)=–0.100,P<0.01;r_(s)=–0.143,P<0.001;r_(s)=–0.082,P<0.05)。结论CA3 mRNA在乳腺癌组织中的低表达与乳腺癌的发生发展及预后存在一定的相关性,有望作为乳腺癌预后潜在标志物,可能与免疫浸润有关。Objective To analyze the relationship between the expression of carbonic anhydrase 3(CA3)in breast cancer tissues,its prognostic potential and the number of immune cells by a variety of online databases.Methods GEPIA2.0 and TIMER databases were used to analyze the difference of CA3 mRNA expression in breast cancer tissues.Bc-GenExMinerv4.7 database was used to analyze the difference of CA3 mRNA expression in breast cancer subcategories.Kaplan-Meier plotter,Bc-GenExMinerv4.7 and PrognoScan databases were used to analyze the effect of CA3 mRNA expression levels on prognosis of patient.LinkedOmics database was used to analyze of the biological behavior involved in CA3 co-expressed genes.TIMER database was used to analyze the relationship between CA3 mRNA expression and immune cells infiltration in breast cancer tissues.Results The expression of CA3 mRNA in breast cancer tissues was lower than that in normal breast tissues(P<0.05),and the expression levels of CA3 mRNA were higher in ER negative(P<0.05),PR negative(P<0.05),HER2 negative(P<0.05)and no lymphatic metastasis(P<0.05).In addition,the expression level of CA3 in breast cancer patients with high Ki67 expression was lower(P<0.05)and closely related to SBR and NPI grade(P<0.05).Breast cancer patients with low expression of CA3 mRNA had lower overall survivall,recurrence free survival,and disease free survival(P<0.05).Ten of the top 50 positively correlated co-expressed genes screened out had low risk ratio (P<0.05), and 11 of the top 50 negatively correlated co-expressed genes screened out had high riskratio (P<0.05). The expression of CA3 mRNA was positively correlated with CD4^(+)T cells and CD8^(+)T cells in breastcancer tissues (r_(s)=0.175, P<0.001;r_(s)=0.137, P<0.001), and negatively correlated with T cell failure marker_(s) LAG3, TIM-3and PVRL2 (r_(s)=–0.100, P<0.01;r_(s)=–0.143, P<0.001;r_(s)=–0.082, P<0.05). Conclusions     The low expression of CA3 mRNAin breast cancer tissues is correlated with the occurrence, development and prognosis of bre

关 键 词：乳腺癌 碳酸酐酶3 预后 免疫浸润 生物信息学 

分 类 号：R737.9[医药卫生—肿瘤] Q811.4[医药卫生—临床医学]