TaARPC5 is required for wheat defense signaling in response to infection by the stripe rust fungus  

作　　者：Jia Guo Huan Peng Tuo Qi Sanding Xu Md Ashraful Islam Brad Day Qing Ma Zhensheng Kang Jun Guo 

机构地区：[1]State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection,Northwest A&F University,Yangling 712100,Shaanxi,China [2]Department of Plant,Soil and Microbial Sciences,Michigan State University,East Lansing,MI 48824,USA

出　　处：《The Crop Journal》2022年第1期88-97,共10页作物学报（英文版）

基　　金：supported by the National Transgenic Key Project of the Ministry of Agriculture of China(2020ZX08009-15B);the National Natural Science Foundation of China(31972224);the National Key Research and Development Program of China(2018YFD0200402);the Natural Science Basic Research Programof Shaanxi(2020JZ-13);the 111 Project from the Ministry of Education of China(B07049);the Open Project Program of State Key Laboratory of Crop Stress Biology for Arid Areas(CSBAA2020010)。

摘　　要：Numerous studies using a combination of confocal microscopic-and pharmacological-based approaches have demonstrated that the actin cytoskeleton dynamically responds to pathogen infection.Here,we observed that phalloidin treatment induced actin nucleation,resulting in enhanced resistance of wheat against the stripe rust pathogen Puccinia striiformis f.sp.tritici(Pst).To define the mechanism underpinning this process,we characterized a family of conserved actin-binding proteins,the actin related protein(ARP)family,which controls actin polymerization.Specifically,we identified and characterized a wheat ARPC gene(Ta ARPC5),which encodes a 136-amino acid protein containing a P16-Arc domain,the smallest subunit of the ARP2/3 complex.Ta ARPC5 m RNA accumulation was induced following the infection of plants with the avirulent Pst strain,and following the elicitation with flagellin(e.g.,flg22)as well.Subcellular localization analysis revealed that Ta ARPC5 is primarily localized to the cortical actin cytoskeleton,and its precise cellular localizations suggest the proximity to processes correlated with the actin-organelle interface.Upon treatment with virulent Pst,Ta ARPC5-knockdown plants exhibited a significant reduction in the expression of PTI-specific m RNAs.Conversely,we observed enhanced induction of reactive oxygen species(ROS)accumulation and a decrease in Ta CAT1 expression following infection with an incompatible Pst isolate.Together with yeast complementation assays,the current study demonstrates a role for Ta ARPC5 in resistance signaling in wheat against Pst infection by regulating the host actin cytoskeleton.

关 键 词：ARPC5 CYTOSKELETON WHEAT Puccinia striiformis f.sp.tritici Virus-induced gene silencing(VIGS) 

分 类 号：S435.121.42[农业科学—农业昆虫与害虫防治]