矮牵牛F3’5’H基因在花瓣呈色中的作用解析  

Identification of F3’5’H Gene in Petunia and the Roles in Petal Coloration

作　　者：符真珠[1] 王锐 张泰然 王慧娟[1] 高杰 李艳敏[1] 蒋卉[1] 王利民[1] 袁欣 李彦邦 张和臣[1] FU Zhenzhu;WANG Rui;ZHANG Tanran;WANG Huijuan;GAO Jie;LI Yanmin;JIANG Hui;WANG Limin;YUAN Xin;LI Yanbang;ZHANG Hechen(Horticultural Research Institute,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China;School of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai 200240,China)

机构地区：[1]河南省农业科学院园艺研究所,河南郑州450002 [2]上海交通大学农业与生物学院,上海200240

出　　处：《河南农业科学》2021年第12期121-127,共7页Journal of Henan Agricultural Sciences

基　　金：河南省自然科学基金项目(212300410360);浦江人才计划项目(19PJ1406700)。

摘　　要：为了揭示F3’5’H基因对矮牵牛花瓣的呈色作用,对其进行生物信息学分析和功能验证。结果表明,矮牵牛基因组中共有HF1和HF2两个位点编码F3’5’H基因。不同类型的矮牵牛具有不同的F3’5’H基因型。F3’5’H1^(R27)在第3个内含子上有1个dTPH9转座子的插入,使其产生2个类型的转录本,其中1个转录本的非正常编码是矮牵牛花瓣呈现粉色的主要原因。在矮牵牛M1×R27中超表达不同类型的F3’5’H基因,除F3’5’H1^(R27)外,其他基因型都能正常行使分子功能,促使转基因后代的矮牵牛花瓣呈现紫色。In order to reveal the coloration effect of F3’5’H gene on petunia petals,bioinformatics analysis and functional identification of F3’5’H gene were conducted. The results showed that there were two gene loci of HF1 and HF2 encoding F3’5’H in petunia genome and different species of petunia contained different F3’5’H genotypes. Among them,F3’5’H1 ^(R27) had a dTPH9 transposon insertion in the third intron,which caused two types of transcripts. The abnormal coding of one transcript was the main cause of petunia pink petals. All genotypes could perform molecular functions normally except for F3’5’H1 ^(R27) by overexpressing different types of F3’5’H genes in M1×R27,which promoted the petunia petals of transgenic progeny to be purple.

关 键 词：矮牵牛 F3’5’H 花色 分子机制 

分 类 号：S681.6[农业科学—观赏园艺]