鸡GATA基因家族鉴定及生物信息学分析  

Identification and Bioinformatics Analysis of GATA Gene Family in Chicken

作　　者：孟婕 王闯 杨晴[1] 张贝[1] 李可强[1] 张传生[1] 耿立英[1] 李祥龙[1] MENG Jie;WANG Chuang;YANG Qing;ZHANG Bei;LI Keqiang;ZHANG Chuansheng;GENG Liying;LI Xianglong(Hebei Key Laboratory of Specialty Animal Germplasm Resources Exploration and Innovation,Hebei Normal University of Science&Technology,Qinhuangdao 066004,China)

机构地区：[1]河北科技师范学院河北省特色动物种质资源挖掘与创新重点实验室,河北秦皇岛066004

出　　处：《河南农业科学》2021年第12期163-169,共7页Journal of Henan Agricultural Sciences

基　　金：河北省鸡现代种业科技创新团队优质特色燕山红玉肉鸡种质创制项目(21326303D-5);河北省现代农业产业技术体系蛋肉鸡创新团队遗传资源开发与利用岗位专家项目(HBCT2018150201);河北省教育厅重点项目(ZD2018034);秦皇岛市科学技术研究与发展计划项目(201903B001);河北科技师范学院博士启动基金项目(2018YB002)。

摘　　要：为了解鸡GATA(ChGATA)家族基因特性,利用生物信息学方法对ChGATA基因家族成员进行鉴定,分析其理化性质、染色体定位、基因结构、保守基序、进化关系、蛋白质结构及蛋白质互作关系。结果表明,共鉴定出6个ChGATA基因,不均匀地分布在5条染色体上。根据蛋白质保守结构域命名为ChGATA2—ChGATA6和ChTRPS1。理化性质分析结果显示,ChGATA均为无稳定的亲水蛋白质,且除ChTRPS1外均为碱性蛋白质。二级结构预测分析结果显示,ChGATA主要由无规则卷曲组成。保守结构域分析结果显示,ChGATA均包含ZnF;ATA结构域。系统进化分析结果显示,ChGATA基因家族可分为3个亚群,且同一亚群的基因结构相似、蛋白质三级结构相似、相互作用蛋白质较一致、亲缘关系较近。In order to understand the gene characteristics of chicken GATA(ChGATA) family,the members of ChGATA gene family were identified by bioinformatics method,and their physical and chemical properties,chromosome location,gene structure,conserved motif,evolutionary relationship,protein structure and protein interaction were analyzed. The results showed that a total of six ChGATA genes were identified and unevenly distributed on five chromosomes. They were named ChGATA2—ChGATA6 and ChTRPS1 according to the conserved domains of the proteins. The results of physical and chemical properties analysis showed that ChGATAs were unstable hydrophilic proteins,and all were basic proteins except for ChTRPS1. The results of secondary structure prediction analysis showed that ChGATA was mainly composed of irregular curls. Conservative domain analysis showed that ChGATA contained ZnF_GATA domain. Phylogenetic analysis showed that the ChGATA gene family could be divided into three subgroups,and the gene structure of the same subgroup was similar,the protein tertiary structure was similar,the interacting proteins were consistent,and the genetic relationship was close.

关 键 词：鸡 GATA基因家族 进化分析 基因结构 蛋白质互作 

分 类 号：S831.2[农业科学—畜牧学]