腹主动脉双线与单线缩窄所致大鼠左心室肥厚模型比较  

Comparison of rat left ventricular hypertrophy model induced by double-line and single-line abdominal aortic constriction

作　　者：田龙海[1] 杨龙[1] 叶芸[1] 何炯红[1] 田水[1] 刘会[1] 夏桂玲 杨君 Tian Longhai;Yang Long;Ye Yun;He Jionghong;Tian Shui;Liu Hui;Xia Guiling;Yang Jun(Guizhou Provincial People’s Hospital,Guiyang 550002,Guizhou,China;First Affiliated Hospital of Guizhou Traditional Chinese Medicine,Guiyang 550002,Guizhou,China.)

机构地区：[1]贵州省人民医院,贵州贵阳550002 [2]贵州中医药大学第一附属医院,贵州贵阳550002

出　　处：《贵州医药》2022年第1期6-8,共3页Guizhou Medical Journal

基　　金：国家自然科学基金“牵张刺激心室肌细胞离子通道重构及AT1信号通路的调控作用”(编号:81260040)。

摘　　要：目的比较腹主动脉单次缩窄与双次缩窄所致大鼠左室肥厚模型的病理及病生特点。方法将60只健康成年雄性SD大鼠随机分为三组:双线组(n=20)、单线组(n=20)、对照组(n=20)。双线组行腹主动脉相邻部位2次系线缩窄,单线组行腹主动脉1次系线缩窄、对照组分离腹主动脉模拟手术过程穿线不系线缩窄。建模8周后行超声心动图评价心功能指标,直接测量左室后壁厚度,计算心肌质量指数。结果建模8周后,与对照组比较,双线组和单线组均表现为不同程度进食量少、体重下降、少动、毛光泽度差、呼吸频率快,且双线组症状尤为明显;双线组、单线组体重(WT)明显低于对照组(P<0.05);双线组、单线组左心室射血分数(LVEF)明显低于对照组(P<0.01),且双线组低于单线组(P<0.05);双线组、单线组心脏舒张末期左心室后壁厚度(LVP-Wd)、心室重量指数(心室重量/体重VW/WT)及B型氨基端利钠肽原(NT-proBNP)检查水平明显高于对照组(P<0.01),且双线组高于单线组(P<0.05)。结论双线腹主动脉缩窄术致心力衰竭(HF)症状及左室肥厚(LVH)程度更明显,建立大鼠LVH模型可选择该方法。Objective To compare the pathology and pathological characteristics of rat left ventricular hypertrophy model induced by single and double abdominal aortic constriction.Methods 60 healthy adult male SD rats were randomly divided into 3 groups which were double-line group(P=20),single-line group(P=20),and control group(P=20).After 8 weeks of modeling,echocardiography was used to evaluate cardiac function indexes,and the thickness of the left ventricular posterior wall was directly measured to calculate the myocardial mass index.Results After 8 weeks of modeling,compared with the control group,the two-line group and the single-line group showed different degrees of food intake loss,weight loss,less movement,poor hair gloss,fast breathing frequency,and the two-line group symptoms were particularly obvious.The weight(WT)of the double-line group and single-line group was significantly lower than that of the control group(P<0.05).The left-ventricular ejection fraction(LVEF)of the double-line group and single-line group was significantly lower than that of the control group(P<0.01)and the double-line group was lower than single-line group(P<0.05).The levels of left ventricular posterior wall thickness(LVP-Wd)at the end of diastole,ventricular weight index(ventricular weight/body weight VW/WT)and type B amino terminal natrriuetic peptide in the double-line group and single-line group were significantly higher than those in the control group(P<0.01),and the double-line group was higher than the single-line group(P<0.05).Conclusion The symptoms of heart failure(HF)and degree of left ventricular hypertrophy(LVH)caused by double-line abdominal aortic constriction are more obvious.This method can be chosen to establish a rat LVH model.

关 键 词：大鼠左心室肥厚模型 腹主动脉缩窄术 心力衰竭 

分 类 号：R-332[医药卫生]