Feasibility study on construction of humanized mice with antibiotics combined with fecal bacteria transplantation  

作　　者：Mei-Ling Xu Yu Zhang Ming Sang Pu-Qing Wang 

机构地区：[1]Xiangyang No.1 People's Hospital,Hubei University of Medicine,Xiangyang 441000,China [2]Hubei Clinical Research Center of Parkinson's Disease,Xiangyang 441000,China

出　　处：《Journal of Hainan Medical University》2021年第24期17-20,共4页海南医学院学报（英文版）

基　　金：National Natural Science Foundation of China(No.81703496);Hubei Medical College Fund Project(No.FDFR201615);Postgraduate Science and Technology Innovation Program of Hubei University of Medicine(No.YC2020027)。

摘　　要：Objective:To select a common method of humanized mice and verify its feasibility with the aim to provide atheoretical basis for subsequent fecal bacteria transplantation research.Methods:1.Specific Pathogen Free(SPF)mice were fed with antibiotics mixed with sterilized drinking water for 3 weeks,during which the general situation of mice was recorded.After 3 weeks,the number of microbes in mice's feces was evaluated by Gram staining.2.After antibiotic treatment,mice were transplanted fecal bacteria by gavage for a week.Then,the contents of fecal bacteria in the donor and recipient feces was determined by RT-PCR,to evaluate the colonization effect of fecal bacteria.Results:(1)Antibiotic treatment had an effect on weight,feed and drinking water consumption of mice,and the fecal bacteria in mice decreased obviously.(2)After fecal bacteria transplantation,the Firmicutes,Bacteroidetes and Firmicutes/Bacteroidetes of the mice receiving Donor1 fecal bacteria accounted for 15.4%,112%and 13.7%of the mice receiving Donor2 fecal bacteria,respectively.The Firmicutes,Bacteroidetes and Firmicutes/Bacteroidetes of Donor1 fecal bacteria accounted for 33.7%,104%and 32%of Donor2,respectively.The recipient's gut microbes had the same trend as human donors.Conclusions:These results indicate that antibiotic treatment combined with fecal bacterial transplantation is feasible and safe for humanizing SPF mice.

关 键 词：ANTIBIOTICS Fecal bacteria transplantation SPF Humanized mice 

分 类 号：R73[医药卫生—肿瘤]