胃癌转移相关长链非编码RNA差异表达谱的研究  

Expression profile of long non-coding RNA in metastatic gastric cancer tissues

作　　者：王亚芳 刘向强 章思偲 刘理礼 WANG Yafang;LIU Xiangqiang;ZHANG Sicai;LIU Lili(Department of Radiotherapy,General Hospital of Southern Theater Command,Guangzhou 510010,China;Department of Gastroenterology,General Hospital of Southern Theater Command;Department of Oncology,Second Affiliated Hospital of PLA Air Force Military Medical University)

机构地区：[1]中国人民解放军南部战区总医院放射治疗科,广州510010 [2]中国人民解放军南部战区总医院消化内科 [3]中国人民解放军空军军医大学第二附属医院肿瘤科

出　　处：《山西医科大学学报》2022年第1期7-13,共7页Journal of Shanxi Medical University

基　　金：国家自然科学基金资助项目(81272349);广州市科技计划基金项目(202102080516)。

摘　　要：目的筛选与胃癌转移相关的长链非编码RNA(lncRNA),寻找可预测及逆转胃癌转移相关的候选基因。方法利用高通量lnc RNA芯片技术分别检测3例未发生远处转移胃癌组织和3例发生远处转移胃癌组织中lncRNA及mRNA表达谱的差异,并对差异表达基因进行基因本体论(GO)和京都基因与基因组百科全书(KEGG)分析。结果远处转移胃癌组与无远处转移组相比,有549个lncRNAs及386个mRNAs差异性表达具有统计学意义,其中远处转移胃癌组织中表达升高的lncRNAs共230个,表达升高的mRNAs共208个,表达下降的lncRNAs共319个,表达下降的mRNAs共178个。GO分析显示差异性表达基因的功能主要涉及到肿瘤细胞的生长、代谢、浸润、免疫调节等多种生物过程。KEGG分析显示差异性表达基因的通路主要涉及到细胞因子及其受体相互作用、趋化因子、细胞代谢等信号通路。结论筛选出了胃癌侵袭转移相关的lncRNAs,深入分析这些差异表达lncRNAs的功能及机制可为胃癌侵袭转移寻找出特异性肿瘤标志物及有效治疗靶点。Objective To screen the long non-coding RNA(lncRNA)associated with the metastasis and the invasion of gastric cancer(GC)and search for the candidate genes that can predict and reverse the invasion of GC.Methods The lncRNA microarray was applied to detect the differential expression profile of lncRNA and mRNA between GC tissues with non-metastasis and metastasis.Gene ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)of the differential expression genes(DEGs)were analyzed.Results A total of 549 lncRNAs and 386 mRNAs with significant difference were screened out from metastatic GC tissues,including 230 up-regulated lncRNAs and 319 down-regulated lncRNAs,208 up-regulated mRNAs and 178 down-regulated mRNAs.GO analysis showed that DEGs mainly participate in the biological processes,such as growth,metabolism,infiltration,and immunological regulation of tumor cells.KEGG analysis indicated that the pathways of differential genes are closely related to the cytokine-cytokine receptor interaction,chemokine signaling,primary immunodeficiency and metabolism.Conclusion Differential expression profile of lncRNAs in metastatic GC tissues are successfully identified.Deep analysis of the function and the mechanism of differentially expressed lncRNAs may provide the specific biomarkers and the effective therapy targets for metastatic GC.

关 键 词：胃癌 侵袭转移 长链非编码RNA 芯片 

分 类 号：R735.2[医药卫生—肿瘤]