采用体外染色体畸变试验检测锐钛型纳米二氧化钛的遗传毒性  

In vitro chromosome aberration evaluation of anatase titanium dioxide nanoparticles

作　　者：杜秀明 洪丽玲 徐灵芝 周庆云 DU Xiuming;HONG Liling;XU Lingzhi;ZHOU Qingyun(Testing Center,Shanghai Research Institute of Chemical Industry Co.,Ltd.,Shanghai 200062;Shanghai Institute of Chemical Industry Testing Co.,Ltd.,Shanghai 200062,China)

机构地区：[1]上海化工研究院有限公司,上海200062 [2]上海化工院检测有限公司,上海200062

出　　处：《癌变．畸变．突变》2022年第1期67-71,共5页Carcinogenesis,Teratogenesis & Mutagenesis

摘　　要：目的:按《OECD化学品测试准则473:体外哺乳动物染色体畸变测试》的要求进行试验,检测锐钛型纳米二氧化钛诱发体外培养的哺乳动物细胞染色体畸变的能力,以评价其是否属于致突变物。方法:试验组受试物终浓度分别设置为0.0078、0.0312、0.125、0.5和2 mg/mL,同时设立溶剂对照组和阳性对照组。试验分为有代谢活化系统短时处理组(+S9,4 h)、无代谢活化系统短时处理组(-S9,4 h)和无代谢活化系统连续处理组(-S9,24 h)3种处理方式,将培养的中国仓鼠肺细胞(CHL)暴露于锐钛型纳米二氧化钛混悬液中,染毒相应时间后收获细胞,经低渗固定后,制片并染色。每个浓度组选取300个分散良好的中期分裂相细胞进行染色体畸变分析。结果:锐钛型纳米二氧化钛在0.0078、0.0312、0.125、0.5和2 mg/mL浓度下,无代谢活化系统短时处理组不含裂隙的染色体畸变率分别为1.67%、1.33%、2.00%、1.00%和2.33%,有代谢活化系统短时处理组不含裂隙的染色体畸变率分别为1.33%、1.33%、2.00%、1.67%和2.00%,无代谢活化系统连续处理组不含裂隙的染色体畸变率分别为1.33%、1.67%、2.00%、1.67%和2.33%,与溶剂对照组相比,差异均无统计学意义(P>0.05)。结论:在本实验条件下,锐钛型纳米二氧化钛在0.0078~2 mg/mL浓度范围内,对CHL细胞染色体无致畸作用。OBJECTIVE:According to the“OECD Guidelines for the testing of chemicals 473”,the in vitro mammalian chromosomal aberration test was used to determine whether anatase titanium dioxide nanoparticles could induce chromosome aberrations was genotoxic or not.METHODS:The final concentrations of the test substance were set to be 0.0078,0.0312,0.125,0.5,and 2 mg/mL.In addition,a solvent control group and a positive control group were set up at the same time.The treatment included short-term treatment group with metabolic activation(+S9,4 h),short-term treatment group without metabolic activation(-S9,4 h)and continuous treatment group without metabolic activation(-S9,24 h).The cultured Chinese hamster lung cells(CHL)were exposed to anatase nano-titanium dioxide suspension,and the cells were harvested after a corresponding period of time,treated with hypotonic solution and then fixed.The cells were spread onto slides and stained.For each dose group,300 well-dispersed metaphase cells were selected for chromosomal aberration analysis.RESULTS:At the dose level of 0.0078,0.0312,0.125,0.5 and 2 mg/mL without metabolic activation,the induced chromosome aberration rates(excluding the gap)were 1.67%,1.33%,2.00%,1.00%and 2.33%,respectively.With metabolic activation,the rates were 1.33%,1.33%,2.00%,1.67%and 2.00%,respectively.After 24 hours treatment without metabolic activation,the rates were 1.33%,1.67%,2.00%,1.67%and 2.33%,respectively.Compared with the solvent control group,there was no statistically significant difference observed(P>0.05).CONCLUSION:Under the current test conditions,anatase titanium dioxide nanoparticles showed no evidence of genotoxic potential in the CHL at the tested dose levels of 0.0078-2 mg/mL.

关 键 词：锐钛型纳米二氧化钛 体外哺乳动物细胞染色体畸变试验 中国仓鼠肺细胞 遗传毒性 

分 类 号：R394-33[医药卫生—医学遗传学]