放射治疗后宫颈癌组织内差异表达基因鉴定  

Identification of differentially expressed genes in cervical carcinoma after radiotherapy

作　　者：白静[1] 刘娜 丁力[1] 雷丽[1] 倪瑞[1] 闫少春[2] 邵国[2,3,4] BAI Jing;LIU Na;DING Li;LEI Li;NI Rui;YAN Shao-chun;SHAO Guo(Department of Radiptherapy, Baotou Tumor Hospital,Baotou 014030;Inner Mongolia Key laboratory of Hypoxic Translational Medicine,Baotou Medical College,Baotou 014060;Beijing Key Laboratory of Hypoxic Conditioning Translational Medicine,Xuanwu Hospital,Capital Medical University,Beijing 100053;Institute of Digestive Diseases of Inner Mongolia Autonomous Region,Department of Laboratory Medicine, the Second Affiliated Hospital of Baotou Medical College,Baotou 014030,China)

机构地区：[1]包头市肿瘤医院放射治疗科,内蒙古包头014030 [2]包头医学院内蒙古自治区低氧转化医学重点实验室,内蒙古包头014060 [3]首都医科大学宣武医院低氧适应转化医学北京重点实验室,北京100053 [4]包头医学院第二附属医院内蒙古自治区消化疾病研究所检验科,内蒙古包头014030

出　　处：《基础医学与临床》2022年第2期215-220,共6页Basic and Clinical Medicine

基　　金：国家自然科学基金(81660307,82060337);内蒙古自然科学基金(2018MS08052,2020MS08010);包头医学院基金(BYJJ-YF201717)。

摘　　要：目的研究放射治疗对宫颈癌组织基因表达的影响,探讨差异表达基因对放射治疗敏感及在抗拒中的作用。方法患者接受相同的放疗模式:全盆腔外照射总剂量45戈瑞(Gy),共25次(45 Gy/25 f),腔内近距离内照射每次剂量5~6 Gy,共治疗5~6次。分别在近距离放射治疗前、近距离放射治疗中、近距离放射治疗结束时留取标本。高通量分析近距离照射前,照射中和照射后基因表达的情况;real-time PCR及Western blot验证与放射敏感和耐受相关的基因。结果生物信息学显示DNA损伤基因表达增加(P<0.05),real-time PCR及Western blot分析DNA损伤相关基因GADD45表达较治疗前增加(P<0.05)。结论放射治疗可引起众多宫颈癌组织基因表达变化,其中GADD45表达增加可能在放射治疗中起到重要作用。Objective To study the effect of radiotherapy on gene expression in cervical cancer tissues and to find the role of differentially expressed genes in sensitivity and resistance to radiotherapy.Methods The patients all received the same radiotherapy as follows:total pelvic external radiation 45 Gy/25 f,intracavitary brachytherapy 5-6 Gy/f with total dosage as 5-6 f.The specimens were collected before treatment and by the end of treatment.High-throughput analysis of gene expression was performed before,during and after irradiation;real-time PCR and Western blot was applied to identify the genes related to radiation sensitivity and tolerance.Results Bioinformaticsanalysis showed an increased expression of DNA damage genes.The expression of DNA damage related genes GADD45 was increased as found by real-time PCR and Western blot.Conclusions Radiotherapy changes the gene expression in cervical cancer tissues and the increase of GADD45 expression may play an important role in radiotherapy.

关 键 词：宫颈癌 放射治疗 生物信息学 

分 类 号：R737.33[医药卫生—肿瘤]