干扰ADAM17抑制人宫颈癌细胞系HeLa细胞增殖  

Interference with ADAM17 inhibits proliferation of human cervical cancer cell line HeLa

作　　者：张春利 齐结华[1] 宦宇[1] 吴守乐[1] 蔡徐山[1] ZHANG Chun-li;QI Jie-hua;HUAN Yu;WU Shou-le;CAI Xu-shan(Department of Clinical Laboratory, Maternal and Child Health Hospital of Jiading District, Shanghai 201821, China)

机构地区：[1]上海市嘉定区妇幼保健院检验科,上海201821

出　　处：《基础医学与临床》2022年第2期255-259,共5页Basic and Clinical Medicine

基　　金：上海市嘉定区卫健委资助项目(2017-QN-06)。

摘　　要：目的探讨干扰去整合素-金属蛋白酶17(ADAM17)对人宫颈癌细胞系HeLa细胞增殖的影响,并对其作用机制进行初步探索。方法在HeLa细胞中转染ADAM17干扰质粒,荧光定量PCR和Western blot验证转染效率;CCK-8法检测细胞增殖,划痕实验和Transwell小室法检测细胞迁移及侵袭;Western blot检测ADAM17、PCNA、EGFR、p-EGFR、ERK和p-ERK蛋白表达。结果转染后HeLa细胞中ADAM17表达被抑制(P<0.05);干扰ADAM17不影响宫颈癌细胞迁移和侵袭,但显著降低了细胞的增殖(P<0.05);增殖相关蛋白PCNA表达水平和p-EGFR、p-ERK表达水平降低(P<0.05)。结论干扰ADAM17可抑制人宫颈癌细胞系HeLa增殖,其作用机制可能与调控EGFR/ERK信号通路有关。Objective To investigate the effect of ADAM17 on proliferation of cervical cancer cells in vitro and its mechanism.Methods The specific shRNA against ADAM17 was transfected into HeLa cells.Western blot and RT-qPCR were used to examine the interference efficiency.The relative rates of proliferation were determined by CCK-8 assay.The migration and invasion were evaluated by scratch test and Transwell test.Western blot was used to detect the protein expression of PCNA,EGFR,p-EGFR,ERK and p-ERK in each group.Results The expression of ADAM17 mRNA and protein were decreased in HeLa cells transfected by sh-ADAM17(P<0.05).ADAM17 had no effect on migration and invasion of cervical cancer cells,but ADAM17 interference significantly inhibited the proliferation of cervical cancer HeLa cells(P<0.05).The expression of PCNA,p-EGFR and p-ERK protein was down-regulated(P<0.05).Conclusions Interfering with ADAM17 can inhibit the proliferation of human cervical cancer cell line HeLa,which may be related to EGFR/ERK signaling pathway.

关 键 词：宫颈癌 去整合素-金属蛋白酶17 增殖 

分 类 号：R737.33[医药卫生—肿瘤]