下调lncRNA DNM3OS通过靶向miR-193a-3p增强人直肠癌细胞系SW-480的放疗敏感性  

Down-regulation of lncRNA DNM3OS enhances radiotherapy sensitivity of human rectal cancer cell line SW-480 by targeting miR-193a-3p

作　　者：刘明胜[1] 陈文超 蔡颖畅 LIU Ming-sheng;CHEN Wen-chao;CAI Ying-chang(Department of Anorectal Surgery, Quzhou People's Hospital, Quzhou 324000, China)

机构地区：[1]衢州市人民医院肛肠外科,浙江衢州324000

出　　处：《基础医学与临床》2022年第2期260-267,共8页Basic and Clinical Medicine

基　　金：衢州市指导性科技计划项目(20172005)。

摘　　要：目的探讨lncRNA DNM3OS对直肠癌SW-480细胞的增殖、凋亡以及放射敏感性的影响及分子机制。方法选取30例直肠癌患者癌组织及癌旁组织,RT-qPCR检测DNM3OS和miR-193a-3p的表达水平;将抑制DNM3OS的表达载体、过表达miR-193a-3p载体转染至SW-480细胞,将DNM3OS与抑制miR-193a-3p的表达载体共转染至SW-480细胞,并用4 Gy射线照射;细胞集落形成实验检测细胞放射敏感性;四甲基偶氮唑盐比色法(MTT)检测SW-480细胞增殖抑制率;流式细胞测量术检测SW-480细胞凋亡;双荧光素酶报告基因实验检测DNM3OS和miR-193a-3p的靶向调控。结果直肠癌组织中DNM3OS表达水平高于癌旁组织,miR-193a-3p表达水平低于癌旁组织(P<0.05)。抑制DNM3OS表达或过表达miR-193a-3p后,SW-480细胞增殖抑制率和凋亡率升高(P<0.05);经射线照射后细胞存活分数降低,细胞增殖抑制率和凋亡率升高(P<0.05)。DNM3OS靶向调控miR-193a-3p,干扰miR-193a-3p表达逆转了抑制lncRNA DNM3OS表达对直肠癌SW-480细胞增殖、凋亡及放射敏感性的影响。结论抑制lncRNA DNM3OS表达可通过靶向上调miR-193a-3p抑制SW-480细胞增殖,促进细胞凋亡以及增强细胞的放射敏感性。Objective To explore the effect of lncRNA DNM3OS on the proliferation,apoptosis and radio-sensitivity of rectal cancer SW-480 cells and its molecular mechanism.Methods The samples from cancerous tissues and adjacent tissues of 30 rectal cancer patients were selected and the expression of DNM3OS and miR-193a-3p was detected by RT-qPCR;Transfected the inhibited DNM3OS expression vector or over-expression miR-193a-3p vector into SW-480 cells and co-transfect DNM3OS and inhibited miR-193a-3p expression vector into SW-480 cells and then irradiated them with 4 Gy rays;Cell colony formation experiment was applined to detect cell radio-sensitivity;methyl thiazolyl tetrazolium assay(MTT)was used to detect cell proliferation inhibition rate;flow cytometry was used to detect cell apoptosis;The targeted regulation of DNM3OS and miR-193a-3p were examined by dual luciferasereporter gene assay.Results The expression of DNM3OS in rectal cancer tissue was higher than that in adjacent tissues and the expression of miR-193a-3p was lower than that in adjacent tissues(P<0.05).After inhibiting DNM3OS expression or over-expression of miR-193a-3p,SW-480 cell proliferation inhibition rate and apoptosis rate was increased(P<0.05);after radiation,cell survival score was decreased and cell proliferation inhibition rate as well as apoptosis rate were increased(P<0.05).DNM3OS targeted and regulated miR-193a-3p,and interference with miR-193a-3p expression reversed the effect of inhibiting lncRNA DNM3OS expression on the proliferation,apoptosis and radiosensitivity of rectal cancer SW-480 cells.Conclusions Down-regulation of expression of lncRNA DNM3OS can inhibit the proliferation of SW-480 cells by up-regulation of miR-193a-3p,thus to promote cell apoptosis and enhance cell radio-sensitivity.

关 键 词：lncRNA DNM3OS miR-193a-3p 直肠癌 放射敏感性 

分 类 号：R735.3[医药卫生—肿瘤]