基于7T动物磁共振成像研究改良Allen’s法制作脊髓外伤动物模型之细胞过度凋亡机制  

Study on the mechanism of excessive apoptosis in animal model of spinal cord injury by modified Allen’s method based on 7T animal magnetic resonance imaging

作　　者：陈溢润 李沐[2] 肖徽荃 CHEN Yi-run;LI Mu;XIAO Hui-quan(Department of critical medicine,the Second Affiliated Hospital of Medical College of Shantou University,Shantou 515041,China;不详)

机构地区：[1]汕头大学医学院第二附属医院重症医学科,广东汕头515041 [2]汕头大学医学院第二附属医院神经外科,广东汕头515041 [3]汕头大学医学院,广东汕头515041

出　　处：《中国处方药》2022年第1期15-17,共3页Journal of China Prescription Drug

基　　金：广东省医学科学技术研究基金项目(B2019127);广东省大学生自主创新训练项目(201910560127)。

摘　　要：目的本研究拟采用改良Allen’s法制作SCI动物模型,通过检测致伤脊髓组织miR-16的表达探究SCI后细胞过度凋亡的可能机制。方法36只成年SD大鼠随机分为正常组(12只)、假手术组(12只)、模型组(12只),通过改良Allen’s制作SCI模型,观察各组行为学评分(BBB评分)、病理表现及PCR法检测miR-16的表达。结果模型组在术后3 d及7 d的BBB评分与正常组和假手术组对比差异有统计学意义(P<0.01)。模型组中的miR-16表达较正常组和假手术组显著升高(P<0.01)。结论研究认为miR-16参与改良Allen’s法制作大鼠SCI模型后引发神经细胞过度凋亡机制。Objective Establish the modified Allen’s-inducted animal spinal cord injury(SCI)model,to explore the possible mechanism of excessive apoptosis of cell after SCI by detecting the expression of miR-16 in injured spinal cord tissue.Methods 36 healthy adult SD rats were randomly divided into 3 groups:normal control group(12),sham operation group(12)group and model group(12).The SCI animal model was established by modified Allen’s.And then observed the Basso,Beattie&Bresnahan locomotor rating scale(BBB scale),pathological manifestations,and the expression of miR-16 via Real-time PCR.Results The BBB scale of the model group on 3 d and 7 d after operation were statistically significant compared with the normal group and sham operation group(P<0.01).Compared with the normal group and sham operation group,the expression level of miR-16 in model group increased.Conclusion We suggest that MiR-16 is involved in the mechanism of excessive apoptosis of nerve cells in modified Allen’s-induced rat SCI model.

关 键 词：脊髓外伤 动物模型 细胞过度凋亡机制 

分 类 号：R-332[医药卫生] R651.2