基于网络药理学探索血府逐瘀汤治疗非小细胞肺癌的作用机制  

Research on the Mechanism of Xuefu Zhuyu Decoctionin the Treatment of NSCLC Based on Network Pharmacology

作　　者：田琳[1] 毕胜男[1] 陈梦竹 牛崇阳 齐文龙[1] 王檀[1] TIAN Lin;BI Shengnan;CHEN Mengzhu;NIU Chongyang;QI Wenlong;WANG Tan(Affiliated Hospital of Changchun University of Chinese Medicine,Changchun 130021,China)

机构地区：[1]长春中医药大学附属医院,吉林长春130021

出　　处：《西部中医药》2022年第1期21-28,共8页Western Journal of Traditional Chinese Medicine

摘　　要：目的:运用中药复方网络药理学和基因芯片探讨血府逐瘀汤治疗非小细胞肺癌(non-smallcell lung carcinoma,NSCLC)的作用机制。方法:利用基因表达数据库(gene expression omnibus,GEO)查找NSCLC的基因芯片,下载NSCLS患者和正常人组织基因并进行差异分析;通过中药系统药理学分析平台(traditional Chinese medicine systems pharmacology database and analysis platform,TCMSP)搜索血府逐瘀汤的活性成分和药物靶点;运用Cytoscape v3.6.0软件,以血府逐瘀汤和NSCLC的交集基因与化合物作为输入数据,构建血府逐瘀汤的中药复方调控网络;利用Cytoscape v3.6.0软件,从深度数据包检测数据库(deep packet inspection,DPI)、蛋白相互作用数据库(biological general repository for interaction datasets,BIOGRID)、人类蛋白参考数据库(human protein reference database,HPRD)等数据库中构建蛋白互作网络(protein-protein interaction networks,PPI);运用R语言进行基因本体(gene ontology,GO)及京都基因与基因组百科全书(kyoto encyclopedia of genes and genomes,KEGG)通路富集分析。结果:从GEO数据库中获取NSCLC与正常组织的差异基因2861个,上调基因1313个,下调基因1548个;获得血府逐瘀汤与NSCLC交集基因69个,候选化合物82个;GO富集分析获得血府逐瘀汤的药物活性成分主要富集在对活性氧的反应过程、营养代谢过程、类固醇激素代谢过程等;血府逐瘀汤治疗NSCLC主要富集在膀胱癌信号通路、糖尿病并发症中的AGE-RAGE信号通路、肿瘤坏死因子信号通路等。结论:网络药理学可以通过建立化学成分、靶点、疾病数据库分析血府逐瘀汤治疗NSCLC的作用机制,为临床使用血府逐瘀汤提供数据支持。Objective:To discuss the mechanism of Xuefu Zhuyu decoction in treating NSCLC using network pharmacology of herbal compounds and gene chip.Methods:GEO database was used to search gene chips of NSCLC,download tissue genes of NSCLC patients and normal person and analyze the differences;TCMSP was used to analyze the active ingredients and drug targets of Xuefu Zhuyu decoction;software Cytoscape v3.6.0 was utilized to construct TCM compound regulation network of Xuefu Zhuyu decoction by taking the intersection genes and compounds of Xuefu Zhuyu decoction and NSCLC as input data;software Cytoscape v3.6.0 was applied to structure PPI protein protein interaction network from DPI,BIOGRID and HPRD databases;R language was used to perform GO and KEGG pathway enrichment analysis.Results:All 2861 differential genes between NSCLC and normal tissues were obtained from GEO database,1313 up-regulated genes and 1548 down-regulated genes;69 intersection genes between Xuefu Zhuyu decoction and NSCLC,and 82 candidate compounds were obtained;GO enrichment analysis showed that the active ingredients of Xuefu Zhuyu decoction were mainly concentrated in the reaction process to reactive oxygen species,nutritional metabolism and steroid hormone metabolism;Xuefu Zhuyu decoction in the treatment of NSCLC were mainly concentrated in AGE-RAGE signaling pathway and tumor necrosis factor signaling pathway in bladder cancer signaling pathway and diabetic complications.Conclusion:Network pharmacology could be used to analyze the mechanism of Xuefu Zhuyu decoction in the treatment of NSCLC via chemical ingredients,targets,disease databases,and provide data support for clinical use of Xuefu Zhuyu decoction.

关 键 词：非小细胞肺癌 血府逐瘀汤 作用机制 网络药理学 

分 类 号：R734[医药卫生—肿瘤]