钛表面高强度仿生形貌涂层构建及生物安全性评估  

Construction of high-strength biomimetic topography coating on titanium surface and evaluation of its biological safety

作　　者：牛强 王潇懿 张周阳 宁小娜 郭维维 张羽博翰 吕前欣 王乐 刘富伟 张浚睿 NIU Qiang;WANG Xiaoyi;ZHANG Zhouyang;NING Xiaona;GUO Weiwei;ZHANG Yubohan;LV Qianxin;WANG Le;LIU Fuwei;ZHANG Junrui(State Key Laboratory of Military Stomatology&National Clinical Research Center for Oral Diseases&Shaanxi Clinical Research Center for Oral Diseases,Department of Oral and Maxillofacial Surgery,School of Stomatology,The Fourth Military Medical University,Xi'an 710032,China;Department of Ophthalmology,Tangdu Hospital,The Fourth Military Medical University,Xi'an)

机构地区：[1]军事口腔医学国家重点实验室,国家口腔疾病临床医学研究中心,陕西省口腔疾病临床医学研究中心,第四军医大学口腔医院颌面外科,西安710032 [2]第四军医大学唐都医院

出　　处：《实用口腔医学杂志》2022年第1期18-23,共6页Journal of Practical Stomatology

基　　金：军事口腔医学国家重点实验室自主研究课题(编号:2020ZB02)。

摘　　要：目的:在钛表面构建高强度,具有微、纳米级梯度仿生形貌涂层,并评估其生物安全性。方法:采用酸蚀及磁控溅射技术构建微、纳米级梯度仿生形貌涂层;扫描电镜检验涂层构建效果;纳米划痕试验检测涂层强度;SD大鼠皮肤刺激试验,毒性、溶血试验及血清生化参数评估该涂层生物安全性。结果:扫描电镜显示该涂层具有微米级、纳米级梯度仿生形貌;纳米划痕结果显示该涂层强度达100 N;生物安全性检测结果显示该涂层不引起皮肤刺激与迟发型超敏反应,无全身毒性,新型涂层组大鼠和光滑组大鼠体重分别为(423.3±11.2)g和(429±10.07)g(P>0.05),实验组与对照组溶血率分别为3.51%±1.04%和2.90%±0.65%(P>0.05)。结论:该研究制备的钛表面仿生形貌涂层,可兼顾临床应用中对涂层强度与生物安全性的双重要求。Objective:To construct a high-strength coating with micron and nanometer gradient bionic morphology on titanium surface,and to evaluate its biological safety.Methods:Micro-and nano-scale gradient bionic topography coating on titanium surface was constructed by acid etching and magnetron sputtering techques.The coating was observed under scanning electron microscope(SEM);the strength of the coating was examined by nano-scratch test.The biological safety of the coating was evaluated by skin irritation,delayed hypersensitivity,systemic toxicity,hemolysis test system and serum biochemical test is SD rats.Results:SEM showed that the coating has a micro-level and nano-level gradient bionic morphology;nano-scratch test results showed that the strength of the coating exceeds 100 N.Biological safety tests showed that the coating did not cause skin irritation and delayed-type hypersensitivity,systemic toxicity,the weight of the rats in the new coating and control group was(423.3±11.2)g and(429±10.07)g(P>0.05),and the hemolysis rate was 3.51%±1.04%and 2.90%±0.65%(P>0.05),respectively.Conclusion:The biomimetic topography coating prepared by combined acid etching and magnetron sputtering technology can meet the dual requirements of coating strength and biological safety for clinical applications.

关 键 词：钛种植体 高强度涂层 仿生形貌 生物安全性 

分 类 号：R783.1[医药卫生—口腔医学]