低能量激光疗法联合Dycal用于大鼠磨牙直接盖髓术的体内研究  

Low level laser therapy combined with dycal as a direct pulp capping meteral in rat molars

作　　者：赵举红 仵楠 崔灵欣 张超杰 祖木热提古丽·阿不来提 徐江[1] ZHAO Juhong;WU Nan;CUI Lingxin;ZHANG Chaojie;Zumuretiguli Abulait;XU Jiang(The First Affiliated Hospital,School of Medicine,Shihezi University,832000,China)

机构地区：[1]石河子大学第一附属医院口腔科,832000

出　　处：《实用口腔医学杂志》2022年第1期24-29,共6页Journal of Practical Stomatology

基　　金：国家自然科学基金(编号:81560442)。

摘　　要：目的:观察低能量激光疗法(LLLT)联合Dycal用于大鼠磨牙直接盖髓对牙髓炎、修复性牙本质形成及IL-1β、TRPA1表达的影响。方法:64只大鼠随机均分成对照组(NC)、低能量激光组(LLLT)、Dycal组(Dy)、联合组(LLLT+Dy)(n=16)。直接盖髓术后3、7、14、28 d取标本,行HE及免疫组化(IHC)染色观察。结果:LLLT组与LLLT+Dy组炎症反应在术后14、28 d都存在显著性差异(P<0.0167);修复性牙本质桥形成在术后14 d,LLLT+Dy组与LLLT组、Dy组存在显著性差异(P<0.0167);术后28 d,LLLT+Dy组与LLLT组存在显著性差异(P<0.0167)。IL-1β、TRPA1阳性MOD值在术后7 d存在显著性差异,Dy组>LLLT组>LLLT+Dy组>NC组(P<0.05),术后14 d存在显著性差异,LLLT组、Dy组均>LLLT+Dy组(P<0.05)。结论:低能量激光联合Dycal可能通过减少IL-1β、TRPA1的表达来减轻牙髓炎症、促进修复性牙本质的形成。Objective:To investigate the pulp reactivity,the restorative dentin formation and the exprssion of IL-1β and TRPA1 after direct pulp capping by low level laser therapy(LLLT)combined with Dycal(Dy)in rat molars.Methods:64 SD rats were randomly divided into 4 groups(n=16):Normal Control group(NC group),LLLT+Dy group,LLLT group and Dy group.Samples were collected on the 3rd,7th,14th and 28th day after pulp capping.HE and immunohistochemical staining(IHC)were performed in different time.Results:There was a significant difference in pulp inflammatory response between LLLT and LLLT+Dy groups on 14th and 28th day after operation(P<0.0167).About reparative dentin formation,there was a significant difference between LLLT+Dy,Dy and LLLT groups(P<0.0167)on the 14th day,between LLLT+Dy and LLLT groups on the 28th day(P<0.0167).IL-1β and TRPA1 expression of group Dy>LLLT>LLLT+Dy>ZC(P<0.05)on the 7th day;group Dy and LLLT>LLLT+Dy>ZC(P<0.05)on the 14th day.Conclusion:LLLT with Dycal for direct pulp capping may reduce the expression of IL-1βand TRPA1 to alleviate pulp inflammation and promore the restorative dentin formation.

关 键 词：低能量激光疗法 DYCAL 直接盖髓术 白介素1Β 瞬时受体电位通道蛋白1 

分 类 号：R783.1[医药卫生—口腔医学]