血清表面增强拉曼光谱检测在SD大鼠舌癌诊断中的应用  

Surface-enhanced Raman spectroscopy of serum for the diagnosis of tongue cancer in SD rats

作　　者：刘洋[1] 孙荣 战德松[3] 张文洁 权慧欣 LIU Yang;SUN Rong;ZHAN Desong;ZHANG Wenjie;QUAN Huixin(Department of Stomatology,The First Affiliated Hospital of Dalian Medical University,116600,China;The Second Affiliated Hospital of Xiamen Medical College;Department of Material Science,School of Stomatology,China Medical University,Liaoning Institute of Stomatology,Shenyang)

机构地区：[1]大连医科大学附属第一医院口腔科,116600 [2]厦门医学院附属第二医院 [3]中国医科大学口腔医学院口腔材料教研室,辽宁省口腔医学研究所

出　　处：《实用口腔医学杂志》2022年第1期47-51,共5页Journal of Practical Stomatology

基　　金：辽宁省教育厅高等学校基本科研项目(编号:LQ2017010);辽宁省自然基金指导计划(编号:2019-ZD-0630)。

摘　　要：目的:评价血清表面增强拉曼光谱(SERS)检测技术在舌癌诊断中的应用价值。方法:建立SD大鼠舌癌模型(n=18),采集舌癌大鼠和无癌健康大鼠(n=15)血清样本,共焦显微拉曼光谱仪检测获取SERS。Origin8.0软件数据预处理,SPSS 23.0软件数据分析。结果:SERS对舌癌血清的判别灵敏度为94.4%,特异度93.3%。结论:SERS技术能准确区分舌癌大鼠血清和正常大鼠血清。Objective:To evaluate the value of Surface-enhanced raman spectroscopy(SERS)of serum in the diagnosis of tongue cancer.Methods:Serum samples from 18 tongue cancer bearing SD rats and 15 healthy controls were examined by confocal microscopic Raman spectrometer,the SERS was obtained and analyzed by Origin 8.0 and SPSS 23.0.Results:The sensitivity and specificity of the SERS for tongue cancer serum were 94.4%and 93.3%respectively.Conclusion:SERS can distinguish the serum from the tongue cancer bearing rats from the healthy controls.

关 键 词：舌癌 微创诊断 血清 早期诊断 表面增强拉曼光谱 

分 类 号：R739.8[医药卫生—肿瘤]