CAPN14通过促进CDK5蛋白的降解在口腔鳞癌中发挥抑癌作用  

CAPN14 inhibits oral squamous cell carcinoma by promoting the degradation of CDK5 protein

作　　者：穆磊 张红[2] 段晓波 陈晓涛[4] MU Lei;ZHANG Hong;DUAN Xiaobo;CHEN Xiaotao(Department of Stomatology,The Eighth Affiliated Hospital of Xinjiang Medical University,Wulumuqi 830014,China;Department of Physical Examination,The Eighth Affiliated Hospital of Xinjiang Medical University,Wulumuqi 830014,China;Department of General Stomatology,West China Hospital of Stomatology,Sichuan University,Chengdu;Department of Stomatology,People's Hospital of Xinjiang Uygur Autonomous Region,Urumqi)

机构地区：[1]新疆医科大学第八附属医院口腔科,乌鲁木齐830014 [2]新疆医科大学第八附属医院体检科,乌鲁木齐830014 [3]四川大学华西口腔医院全科 [4]新疆维吾尔自治区人民医院口腔科

出　　处：《实用口腔医学杂志》2022年第1期111-115,共5页Journal of Practical Stomatology

摘　　要：目的:探究钙激活中性蛋白酶14(CAPN14)在口腔鳞癌(OSCC)中的生物学效应及作用机制。方法:生物信息学方法分析CAPN14在OSCC细胞系SCC9、SCC25和CAL27中的临床特征及相互作用分子,慢病毒转染技术将CAL27细胞分为对照组、CAPN14过表达组及回复组。实时荧光定量PCR(qPCR)及Western Blot检测CAPN14和CDK5的mRNA及蛋白表达;CCK-8实验检测细胞增殖能力;Transwell侵袭实验检测细胞侵袭能力。结果:相比癌旁组织,OSCC组织中CAPN14的表达含量下降(P<0.01)。相比对照组,过表达组CAL27细胞中CDK5的蛋白表达水平降低,CDK5蛋白半衰期缩短,细胞增殖及转移能力减弱(P<0.01);而相比过表达组,回复组细胞CDK5的蛋白表达水平增高,细胞增殖及转移能力增强(P<0.01)。结论:CAPN14可促进CDK5蛋白的降解,并抑制OSCC细胞的恶性增殖及转移能力。Objective:To investigate the biological effects and the mechanism of calcium-activated neutral protease 14(CAPN14)in oral squamous cell carcinoma(OSCC).Methods:The clinical features and interaction molecules of CAPN14 in OSCC cell lines of SCC9,SCC25 and CAL27 were analyzed by bioinformatics.CAL27 cells were used as the cell model and were divided into control group,CAPN14 overexpression group and rescue group by lentiviral transfection.The mRNA and protein expressions of CAPN14 and CDK5 were detected by qPCR and Western blot.The cell proliferation was detected by CCK-8 assay;and the invasion ability was detected by Transwell invasion test.Results:Compared with the para-cancerous tissues,the expression of CAPN14 in OSCC was lower(P<0.01).Compared with the control cells,the protein expression level of CDK5 in the CAPN16 overexpressed CAL27 cells was decreased,the half-life of CDK5 protein was shortened,and the cell proliferation and invasion ability were decreased(P<0.01);the expression level of CDK5 protein in the rescue group was increased,and the cell proliferation and invasion ability were increased(P<0.01).Conclusion:CAPN14 can promote the degradation of CDK5 protein and inhibit the proliferation and invasion of OSCC cells.

关 键 词：口腔鳞癌 CDK5 CAPN14 增殖 肿瘤转移 

分 类 号：R739.8[医药卫生—肿瘤]