FPS-ZM1和mNGF对癫痫幼鼠脑内HMGB1和TRX表达的影响  

Effect of FPS-ZM1 and mNGF on the Expression of HMGB1 and TRX in the Brain of Young Rats with Epilepsy

作　　者：李冬梅[1] 陈巧彬[1] 陈琅[1] 方琼[1] LI Dongmei;CHEN Qiaobin;CHEN Lang;FANG Qiong(Department of Pediatrics,Fujian Provincial Hospital,Provincial Clinical College of Fujian Medical University,Fuzhou 350001,China)

机构地区：[1]福建医科大学省立临床医学院,福建省立医院儿科,福州350001

出　　处：《福建医科大学学报》2021年第6期513-516,共4页Journal of Fujian Medical University

基　　金：福建省医学创新课题(2019-CXB-16)。

摘　　要：目的研究晚期糖基化终末产物(RAGE)受体阻断剂FPS-ZM1和鼠神经生长因子(mNGF)对戊四氮致痫幼鼠海马区高迁移率族蛋白B1(HMGB1)和硫氧还蛋白(TRX)表达的影响。方法取130只清洁级Wistar雄性大鼠幼崽,随机分为4组,即A组(空白对照组)、B组(癫痫对照组)、C组(mNGF干预组)和D组(FPS-ZM1干预组)。幼鼠腹腔注射戊四氮40 mg/kg建立癫痫模型,建模成功后,4组均连续干预1周,其中A组和B组腹腔注射等剂量生理盐水,C组和D组分别腹腔注射mNGF 4μg/kg、FPS-ZM1 1 mg/kg,干预结束后,各组分别于3、24、72 h时段麻醉后分离海马组织。采用Western-blot方法测定海马HMGB1的含量,ELISA法检测海马TRX的浓度。结果 A组大鼠未见惊厥发作,海马区HMGB1表达水平在各时段均明显低于B组、TRX浓度均高于B组,差别有统计学意义(P<0.001);D组和C组的HMGB1表达水平在各时段均低于B组、TRX浓度均高于B组,差别有统计学意义(P<0.05);D组的HMGB1表达水平在3、24 h时段较C组下降,差别有统计学意义(P<0.05),在72 h时段与C组的差别则无统计学意义(P>0.05)。结论 FPS-ZM1和mNGF具有减轻癫痫幼鼠脑损伤的作用,其机制可能是通过减轻大脑的炎症反应和氧化应激来实现的。Objective To explore the effect of receptor for adcanced glycation end products(RAGE) inhibitor FPS-ZM1 and mouse nerve growth factor(mNGF) on the expression of high mobility group box-1(HMGB1) and thioredoxin(TRX) in the brain of young rats with chronic epilepsy induced by pentylenetetrazole. Methods 130 clean level of male Wistar rats were randomly divided into group A(blank control group), group B(epilepsy control group), and group C(mNGF intervention group),group D(FPS-ZM1 intervention group), the rats were intraperitoneal injected with pentylenetetrazol 40 mg/kg to establish an epilepsy model. After successful modeling, groups A and B were given equal doses of normal saline, group C were given mNGF 4 μg/kg, and group D were given FPS-ZM1 1 mg/kg by intraperitoneal injection for 7 days. The hippocampus was isolated at 3, 24 and 72 h after anesthesia. The Western-blot method was used to detect the expression of HMGB1 in hippocampus, and the ELISA was used to determine and the concentration of TRX. Results There was no seizure in group A,the expression level of HMGB1 in hippocampus was significantly lower than that in group B at different time points, the TRX content was higher than that in group B(P<0.001). The expression level of HMGB1 in group C and group D were lower than those in group B at different time points, the TRX concentration were higher than group B, the difference was statistically significant(P<0.05);The expression level of HMGB1 in group D was decreased at 3 and 24 h compared with group C, the difference was statistically significant(P<0.05);And there was no significant difference between 72 h in this two groups(P>0.05). Conclusion Both FPS-ZM1 and mNGF could reduce the brain injury in young epilepic rats, and its possible mechanism was to reduce the cerebral inflammatory reaction and oxidative stress.

关 键 词：癫痫 FPS-ZM1 鼠神经生长因子 高迁移率族蛋白B1 硫氧还蛋白 

分 类 号：R742.1[医药卫生—神经病学与精神病学]