Fabrication and evaluation of an optimized xenogenic decellularized costal cartilage graft:preclinical studies of a novel biocompatible prosthesis for rhinoplasty  

作　　者：Shuang Lin Yuanjia He Meihan Tao Aijun Wang Qiang Ao 

机构地区：[1]Department of Plastic Surgery,Shengjing Hospital of China Medical University,36 Sanhao Street,Shenyang 11004,China [2]Department of Tissue Engineering,China Medical University,77 Puhe Road,Shenyang 110112,China [3]Department of Stomatology,The Fourth Affiliated Hospital of China Medical University [4]Chongshan East Road,Shenyang 110033,China 4Surgical Bioengineering Laboratory,Department of Surgery,School of Medicine,University of California Davis,Sacramento,CA 95817,USA [5]Institute of Regulatory Science for Medical Device,National Engineering Research Center for Biomaterials,Sichuan University,24 Yihuan Street,Chengdu 610065,China

出　　处：《Regenerative Biomaterials》2021年第6期106-118,共13页再生生物材料（英文版）

基　　金：supported by Sichuan Science and Technology Program(2020YFH0008);National Natural Science Foundation of China(No.81771351);Joint Research Fund Liaoning-Shenyang National Laboratory for Materials Science(2019JH3/30100022);National Key R&D Program of China(2017YFA0105802).

摘　　要：On account of the poor biocompatibility of synthetic prosthesis,millions of rhinoplasty recipients have been forced to choose autologous costal cartilage as grafts,which suffer from limited availability,morbidity at the donor site and prolonged operation time.Here,as a promising alternative to autologous costal cartilage,we developed a novel xenogeneic costal cartilage and explored its feasibility as a rhinoplasty graft for the first time.Adopting an improved decellularization protocol,in which the ionic detergent was substituted by trypsin,the resulting decellularized graft was confirmed to preserve more structural components and better mechanics,and eliminate cellular components effectively.The in vitro and in vivo compatibility experiments demonstrated that the decellularized graft showed excellent biocompatibility and biosecurity.Additionally,the functionality assessment of rhinoplasty was performed in a rabbit model,and the condition of grafts after implantation was comprehensively evaluated.The optimized graft exhibited better capacity to reduce the degradation rate and maintain the morphology,in comparison to the decellularized costal cartilage prepared by conventional protocol.These findings indicate that this optimized graft derived from decellularized xenogeneic costal cartilage provides a new prospective for future investigations of rhinoplasty prosthesis and has great potential for clinical application.

关 键 词：DECELLULARIZATION costal cartilage extracellular matrix PROSTHESIS RHINOPLASTY 

分 类 号：R622[医药卫生—整形外科]