蝎毒素抗菌肽La39的序列分析及功能鉴定  

Functional identification and bioinformation analysis of antimicrobial peptide from scorpion venom

作　　者：朱晨刚 贾庭[1] ZHU Chengang;JIA Ting(School of Biological Sciences,Guizhou Education University,Guiyang 550018,China;College of Life Sciences,Wuhan University,Wuhan 430072,China)

机构地区：[1]贵州师范学院生物科学学院,贵阳550018 [2]武汉大学生命科学学院,武汉430072

出　　处：《生物学杂志》2022年第1期33-36,共4页Journal of Biology

基　　金：国家自然科学基金项目(30500266);贵州师范学院自然科学研究基金项目(12BS025)。

摘　　要：从中国海南省采集澳链尾蝎(Liocheles australasiae),构建蝎毒腺cDNA文库,筛选得到一条抗菌肽基因La39,其成熟肽由18个氨基酸组成,二级结构呈现为α-helix,亲水和疏水氨基酸位于分子的对立面,形成较强的两性拓扑结构。MICs测定实验、溶血实验和抑制菌株生长实验结果表明,La39对金黄色葡萄球菌的最小抑制质量浓度为50μg/mL,对大肠杆菌无抑制活性,有溶血活性。A scorpion gland cDNA library was constructed from the scorpion Liocheles australasiae which was collected from Hainan Province of China.And then an antimicrobial peptide gene,named La39,was screened from the cDNA library.Its mature peptide is composed of 18 amino acids,and its secondary structure isα-helix.Hydrophilic and hydrophobic amino acids are located on the oppo-site side of the molecule,forming a strong amphoteric topological structure.The results of MICs assay,and hemolytic experiments and growth inhibitory assay showed that the minimum inhibition concentration of La39 for Staphylococcus aureus was 50μg/mL,which,however,had no inhibitory activity on Escherichia coli,and had hemolytic activity.

关 键 词：抗菌肽 澳链尾蝎 CDNA文库 活性多肽 

分 类 号：Q74[生物学—分子生物学]