多环芳烃降解菌的筛选及降解特性分析  

Isolation and analysis of degradation ability of PAHs-degrading bacteria tolerant to diverse physical settings

作　　者：甄静[1,2] 李磊 王继雯[1,2] 杨文玲[1,2] 杜志敏[1,2] 李亮亮 巩涛[1,2] ZHEN Jing;LI Lei;WANG Jiwen;YANG Wenling;DU Zhimin;LI Liangliang;GONG Tao(Key Laboratory of Microbial Engineering of Henan,Zhengzhou 450008,China;Institute of Biology Co.,Ltd.,Henan Academy of Sciences,Zhengzhou 450008,China)

机构地区：[1]河南省微生物工程重点实验室,郑州450008 [2]河南省科学院生物研究所有限责任公司,郑州450008

出　　处：《生物学杂志》2022年第1期46-50,共5页Journal of Biology

基　　金：国家自然科学基金项目(No.31800361,32001206);河南省科学院基本科研业务费(200605106,200605009)。

摘　　要：为了获得耐受各种不良环境的多环芳烃(PAHs)降解菌,利用多环芳烃(菲和芘)、重金属(Cu^(2+)、Cr^(6+))以及氮源缺乏培养基从南阳石油污染土壤中分离筛选一株耐受不良环境的菲芘降解菌,通过形态学特征和分子生物学方法鉴定菌株,并研究菌株对重金属Cr^(6+)和Cu^(2+)、氮源缺乏及酸碱的耐受程度,进一步探究在这些不良环境下菌株对多环芳烃菲和芘的降解效果。结果表明,菌株属于根瘤菌属,命名为F3-1。在液体培养基中,菌株F3-1对重金属Cr^(6+)和Cu^(2+)的耐受质量浓度均为20 mg/L;在氮源缺乏的情况下,菌株能够正常生长;菌株F3-1在pH 5.0和pH 7.4环境下均可以正常生长;菌株F3-1在不良环境下对多环芳烃菲和芘均有很好的降解效果,5 d后在pH 7.4条件下对菲(50 mg/L)的降解率为35.54%,在pH 5.0条件下对芘(50 mg/L)的降解率为53.43%。In order to obtain PAHs-degrading bacteria tolerant to diverse physical settings,a high efficient polycyclic aromatic hydro-carbons-degrading bacterial strain was isolated by heavy metal tolerance capacity and lack of nitrogen source culture method from a soil collected from Nanyang petroleum contaminated soil.The strain was identified by morphological characteristics and molecular biological methods,and its tolerance to heavy metals(Cr^(6+)and Cu^(2+)),lack of nitrogen source and acid-base tolerance was studied to further ex-plore the degradation effect of phenanthrene and pyrene under these adverse environmental conditions.The isolated strain was Rhizobi-um pusense,named F3-1.The result showed that the higher tolerance mass concentration of metals(Cr^(6+)and Cu^(2+))of the strain F3-1 was 20 mg/L.The strain F3-1 could grow normally under the condition of absence of nitrogen source,pH 5.0 and pH 7.4.The strain F3-1 could degrade single phenanthrene or single pyrene under extreme conditions.After 5 days,the strain F3-1 could degrade 35.54%of phenanthrene(50 mg/L)at pH 7.4,and the degradation rate of pyrene(50 mg/L)was up to 35.54%at pH 5.0.

关 键 词：重金属 氮源缺乏 菲 芘 降解 根瘤菌 多环芳烃 

分 类 号：Q93[生物学—微生物学] X172[环境科学与工程—环境科学]