Calcium overload and reactive oxygen species accumulation induced by selenium deficiency promote autophagy in swine small intestine  

作　　者：Yingying Zheng Haoyue Guan Jie Yang Jingzeng Cai Qi Liu Ziwei Zhang 

机构地区：[1]College of Veterinary Medicine,Northeast Agricultural University,Harbin,China [2]College of Animal Science and Veterinary Medicine,Southwest Minzu University,Chengdu,Sichuan,China [3]Key Laboratory of the Provincial Education,Department of Heilongjiang for Common Animal Disease Prevention and Treatment,College of Veterinary Medicine,Northeast Agricultural University,Harbin,China

出　　处：《Animal Nutrition》2021年第4期997-1008,共12页动物营养（英文版）

基　　金：supported by the Natural Science Foundation of Heilongjiang Province of China(YQ2021C021)。

摘　　要：Selenium(Se)deficiency can seriously affect the small intestine of swine,and cause diarrhea in swine.However,the specific mechanism of Se deficiency-induced swine diarrhea has rarely been reported.Here,to explore the damage of Se deficiency on the calcium homeostasis and autophagy mechanism of swine,in vivo and in vitro models of swine intestinal Se deficiency were established.Twenty-four pure line castrated male Yorkshire pigs(45 d old,12.50±1.32 kg,12 full-sibling pairs)were divided into 2 equal groups and fed Se-deficient diet(0.007 mg Se/kg)as the Se-deficiency group,or fed Se-adequate diet(0.3 mg Se/kg)as the control group for 16 weeks.The intestinal porcine enterocyte cell line(IPEC-J2)was divided into 2 groups,and cultured by Se-deficient medium as the Se-deficient group,or cultured by normal medium as the control group.Morphological observations showed that compared with the control group,intestinal cells in the Se-deficiency group were significantly damaged,and autophagosomes increased.Autophagy staining and cytoplasmic calcium staining results showed that in the Sedeficiency group,autophagy increased and calcium homeostasis was destroyed.According to the reactive oxygen species(ROS)staining results,the percentage of ROS in the Se-deficiency group was higher than that in the control group in the in vitro model.Compared with the control group,the protein and mRNA expressions of autophagy-calcium-related genes including Beclin 1,microtubule-associated proteins 1 A(LC3-1),microtubule-associated proteins 1 B(LC3-2),autophagy-related protein 5(ATG5),autophagy-related protein 12(ATG12),autophagy-related protein 16(ATG16),mammalian target of rapamycin(mTOR),calmodulin-dependent protein kinase kinaseβ(CAMKK-β),adenosine 5’-monophosphate-activated protein kinase(AMPK),sarco(endo)plasmic reticulum Ca2+-ATPase(SERCA),and calpain in the Se-deficiency group were significantly increased which was consistent in vivo and in vitro(P<0.05).Altogether,our results indicated that Se deficiency could destroy the calcium

关 键 词：Se deficiency swine Small intestine Intestinal porcine enterocyte cell line AUTOPHAGY Ca overload 

分 类 号：S858.28[农业科学—临床兽医学]