云南牛血液标本中哺乳动物正呼肠孤病毒(YNSZ/V207/2017)的分离鉴定  

Isolation and identification of mammalian orthoreovirus(YNSZ/V207/2017)from bovine blood samples in Yunnan

作　　者：李占鸿 肖雷[1] 李卓然 宋子昂 谢佳芮 杨振兴[1] 朱沛 李华春[1] 廖德芳[1] 杨恒[1,3] LI Zhan-hong;XIAO Lei;LI Zhuo-ran;SONG Zi-ang;XIE Jia-rui;YANG Zhen-xing;ZHU Pei;LI Hua-chun;LIAO De-fang;YANG Heng(Yunnan Tropical and Subtropical Virus Diseases Laboratory,Yunnan Animal Science and Veterinary Institute,Kunming 650224,China;College of Veterinary Medicine,Yunnan Agricultural University,Kunming 650201,China;College of Agriculture and Life Sciences,Kunming University,Kunming 650214,China)

机构地区：[1]云南省畜牧兽医科学院,云南省热带亚热带动物病毒病重点实验室,昆明650224 [2]云南农业大学动物医学院,昆明650201 [3]昆明学院农学与生命科学学院,昆明650214

出　　处：《中国人兽共患病学报》2022年第1期1-9,共9页Chinese Journal of Zoonoses

基　　金：国家重点研发计划(No.2017YFC1200505,No.2016YFD0500908);国家自然科学基金(No.31760744);国家公益性行业(农业)专项(No.201303035);云南省中青年学术和技术带头人后备人才培养项目(No.2017HB055)联合资助。

摘　　要：目的掌握云南省动物病毒的多样性和传播风险。方法在云南省师宗县设立10头哨兵牛,定期采血接种细胞进行病毒分离。通过RT-PCR、电镜观察、高通量测序与血清中和试验进行分离病毒的鉴定、全基因组序列获取与流行病学调查。结果2017年从采集的哨兵牛血液样本中分离出2株蓝舌病病毒、3株帕利亚姆病毒、2株流行性出血病病毒和1株待鉴定病毒(YNSZ/V207/2017)。电镜下YNSZ/V207/2017病毒粒子直径约80 nm,呈二十面体对称;全基因组序列分析显示其为哺乳动物正呼肠孤病毒(Mammalian orthoreovirus,MRV)血清1型毒株(MRV-1),病毒的不同基因节段分别与人、白尾鹿、水貂、果子狸和棕蝠上分离MRV的序列相似度最高;血清中和试验表明当地牛、羊和猪中MRV-1的抗体阳性率分别为32.5%、20.0%和42.5%。结论在云南省牛血液标本中分离出1株MRV-1型毒株,该毒株可能为不同宿主来源的基因重配毒株,MRV-1在当地家畜中广泛流行。This study investigated the diversity of viruses and related epidemics in animals in Yunnan Province.Blood samples were collected weekly from ten sentinel cattle in Shizong County,and cells were inoculated for viral isolation.The identity and prevalence of the isolated viruses were confirmed through RT-PCR,electron microscopy observation,whole genome sequencing and serum neutralization testing.Two strains of bluetongue virus,three strains of palyam virus,two strains of epidemic hemorrhagic disease virus and one unidentified virus(YNSZ/V207/2017)were isolated from the blood samples of sentinel cattle.The virion of YNSZ/V207/2017 was approximately 80 nm in diameter and showed icosahedral symmetry under electron microscopy.Whole genome sequence analysis indicated that YNSZ/V207/2017 belonged to mammalian orthoreovirus serotype 1(MRV-1)and shared the highest sequence similarity with the corresponding segments of MRV isolated from humans,Odocoileus virginianus,Mustla vison,Paguma larvata taivana and Eptesicus serotinus.Serum neutralization tests indicated that the positivity rates of MRV-1 antibodies in local cattle,sheep and pigs were 32.5%,20.0%and 42.5%,respectively.In conclusion,an MRV-1 strain isolated from cattle in Yunnan Province may be a gene reassortant strain from different host sources,and the virus was found to be widespread in local livestock.

关 键 词：哺乳动物正呼肠孤病毒 血清型 病毒分离 全基因组测序 基因重配 流行病学调查 

分 类 号：S855.3[农业科学—临床兽医学]