Genome-wide association analysis identified molecular markers associated with important tea flavor-related metabolites  

作　　者：Kaixing Fang Zhiqiang Xia Hongjian Li Xiaohui Jiang Dandan Qin Qiushuang Wang Qing Wang Chendong Pan Bo Li Hualing Wu 

机构地区：[1]Tea Research Institute,Guangdong Academy of Agricultural Sciences,Guangdong Key Laboratory of Tea Plant Resources Innovation&Utilization,Guangzhou 510640,China [2]Institute of Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou 570100,China [3]Hainan University,Haikou 570228,China

出　　处：《Horticulture Research》2021年第1期714-730,共17页园艺研究（英文）

基　　金：This work was supported by Key-Area Research and Development Program of Guangdong Province[2020B020220004];the earmarked fund for the National Modern Agro-industry Technology Research System(CARS-19);the National Natural Science Foundation of China(31600550);Science and Technology program of Guangdong Province(2016A030303032 and 2019B030316026);Discipline team building projects of Guangdong Academy of Agricultural Sciences in the 13th Five-Year Period(201619TD);Special funds for scientific innovation strategy-construction of high level Academy of Agriculture Science(R2017PY-QY009);Integrated demonstration of key techniques for the industrial development of featured crops in rocky desertification areas of Yunnan-Guangxi-Guizhou provinces(SMH2019-2021);the Central Public-interest Scientific Institution Basal Research Fund for Chinese Academy of Tropical Agricultural Sciences(1630052019022).

摘　　要：The characteristic secondary metabolites in tea(theanine,caffeine,and catechins)are important factors contributing to unique tea flavors.However,there has been relatively little research on molecular markers related to these metabolites.Thus,we conducted a genome-wide association analysis of the levels of these tea flavor-related metabolites in three seasons.The theanine,caffeine,and catechin levels in Population 1 comprising 191 tea plant germplasms were examined,which revealed that their heritability exceeded 0.5 in the analyzed seasons,with the following rank order(highest to lowest heritabilities):(+)-catechin>(−)-gallocatechin gallate>caffeine=(−)-epicatechin>(−)-epigallocatechin-3-gallate>theanine>(−)-epigallocatechin>(−)-epicatechin-3-gallate>catechin gallate>(+)-gallocatechin.The SNPs detected by amplified-fragment SNP and methylation sequencing divided Population 1 into three groups and seven subgroups.An association analysis yielded 307 SNP markers related to theanine,caffeine,and catechins that were common to all three seasons.Some of the markers were pleiotropic.The functional annotation of 180 key genes at the SNP loci revealed that FLS,UGT,MYB,and WD40 domain-containing proteins,as well as ATP-binding cassette transporters,may be important for catechin synthesis.KEGG and GO analyses indicated that these genes are associated with metabolic pathways and secondary metabolite biosynthesis.Moreover,in Population 2(98 tea plant germplasm resources),30 candidate SNPs were verified,including 17 SNPs that were significantly or extremely significantly associated with specific metabolite levels.These results will provide a foundation for future research on important flavor-related metabolites and may help accelerate the breeding of new tea varieties.

关 键 词：analysis BREEDING Population 

分 类 号：S571.1[农业科学—茶叶生产加工]