Proteomic analysis of early-stage incompatible and compatible interactions between grapevine and P.viticola  

作　　者：Guo-Tian Liu Bian-Bian Wang David Lecourieux Mei-Jie Li Ming-Bo Liu Rui-Qi Liu Bo-Xing Shang Xiao Yin Li-Jun Wang Fatma Lecourieux Yan Xu 

机构地区：[1]State Key Laboratory of Crop Stress Biology for Arid Areas,College of Horticulture,Northwest A&F University,Yangling,China [2]Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China,Ministry of Agriculture,Northwest A&F University,Yangling,China [3]UMR1287 EGFV,CNRS,Universitéde Bordeaux,INRAE,Bordeaux Sciences Agro,ISVV,Villenave d’Ornon,France [4]Institute of Botany,The Chinese Academy of Sciences,Beijing,China

出　　处：《Horticulture Research》2021年第1期1560-1580,共21页园艺研究（英文）

基　　金：the National Key Research and Development Program of China(2018YFD1000300,2019YFD1002500);National Natural Science Foundation of China(31601716,No.31972374)。

摘　　要：Wild grapevines can show strong resistance to the downy mildew pathogen P.viticola,but the associated mechanisms are poorly described,especially at early stages of infection.Here,we performed comparative proteomic analyses of grapevine leaves from the resistant genotype V.davidii“LiuBa-8”(LB)and susceptible V.vinifera“Pinot Noir”(PN)12h after inoculation with P.viticola.By employing the iTRAQ technique,a total of 444 and 349 differentially expressed proteins(DEPs)were identified in LB and PN,respectively.The majority of these DEPs were related to photosynthesis,respiration,cell wall modification,protein metabolism,stress,and redox homeostasis.Compared with PN,LB showed fewer downregulated proteins associated with photosynthesis and more upregulated proteins associated with metabolism.At least a subset of PR proteins(PR10.2 and PR10.3)was upregulated upon inoculation in both genotypes,whereas HSP(HSP70.2 and HSP90.6)and cell wall-related XTH and BXL1 proteins were specifically upregulated in LB and PN,respectively.In the incompatible interaction,ROS signaling was evident by the accumulation of H_(2)O_(2),and multiple APX and GST proteins were upregulated.These DEPs may play crucial roles in the grapevine response to downy mildew.Our results provide new insights into molecular events associated with downy mildew resistance in grapevine,which may be exploited to develop novel protection strategies against this disease.

关 键 词：METABOLISM HOMEOSTASIS analysis 

分 类 号：Q943.2[生物学—植物学]